应用福尔马林固定石蜡包埋心脏组织中补体成分 C9 免疫组织化学检测食蟹猴（Aotus nancymai）早期心肌细胞死亡：一项回顾性研究。
Detection of early myocardial cell death in owl monkeys (Aotus nancymai) using complement component C9 immunohistochemistry in formalin-fixed paraffin-embedded heart tissues: A retrospective study.
机构信息
Comparative Medicine Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Laboratory of Malaria Immunology and Vaccinology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
出版信息
J Med Primatol. 2022 Apr;51(2):93-100. doi: 10.1111/jmp.12567. Epub 2021 Dec 31.
BACKGROUND
Owl monkeys are commonly used in biomedical research which is affected by the high incidence of cardiomyopathy in this species. Occasionally, owl monkeys with no clinical signs of heart disease are found dead and at necropsy show no, or very mild, cardiomyopathy. A possible explanation for sudden death is acute myocardial infarction; however, early myocardial changes may be difficult to assess by conventional stains and light microscopy.
METHODS
Complement component C9 immunohistochemistry was performed in paraffin-embedded heart tissue samples from owl monkeys who died suddenly, or were euthanized due to sickness, to determine whether these animals suffered from acute myocardial infarcts.
RESULTS AND CONCLUSION
C9 deposits were found in the myocardium of 19 out of 20 (95%) animals. The findings in this study suggest owl monkeys suffer from acute myocardial infarcts, and complement component C9 immunohistochemistry may be a useful diagnostic tool.
背景
猫头鹰猴常用于生物医学研究，而该物种中心肌病的发病率很高。偶尔会发现没有心脏病临床症状的猫头鹰猴突然死亡，尸检时没有或仅有非常轻微的心肌病。突然死亡的一个可能解释是急性心肌梗死;然而，通过常规染色和光镜检查，早期心肌变化可能难以评估。
方法
对因突然死亡或因病安乐死的猫头鹰猴的心脏组织石蜡包埋样本进行补体成分 C9 免疫组化染色，以确定这些动物是否患有急性心肌梗死。
结果与结论
在 20 只动物中的 19 只（95%）心脏组织中发现了 C9 沉积。本研究结果表明，猫头鹰猴患有急性心肌梗死，补体成分 C9 免疫组化染色可能是一种有用的诊断工具。
Zotero 插件