Ugwuja Emmanuel Ike, Erejuwa Omotayo O, Ugwu Nicholas C
Department of Biochemistry, Faculty of Sciences, Ebonyi State University, P. M. B. 053 Abakaliki, Ebonyi State, Nigeria. ; Department of Chemical Pathology, Faculty of Medicine, Ebonyi State University, P. M. B. 053 Abakaliki, Ebonyi State, Nigeria.
Department of Pharmacology and Therapeutics, Faculty of Medicine, Ebonyi State University, P. M. B. 053 Abakaliki, Ebonyi State, Nigeria.
Adv Pharm Bull. 2016 Jun;6(2):271-4. doi: 10.15171/apb.2016.038. Epub 2016 Jun 30.
To determine the potential protective effect of spice mixture containing garlic, ginger and nutmeg on the liver and kidney of cadmium exposed rats.
Male albino rats (n=30) weighing 120 - 180 g, grouped into five (1-5) of 6 rats/group were studied. Group 1 (NC) rats were administered distilled water (1 ml) orally for 4 weeks and served as the negative control while group 2 (PC) rats were administered low dose (LD) cadmium (25 mg/kg body weight) orally for 4 weeks and served as positive control. Group 3 (TBE) rats were treated with spice mixture (SM); 300 mg/kg body weight orally for 2 weeks and then administered LD cadmium for 4 weeks. While group 4 (CET) rats were concurrently administered LD cadmium and SM for 4 weeks, group 5 (TAE) rats were administered LD cadmium for 4 weeks and then treated with SM for 2 weeks. The whole experiment lasted for 42 day after which the animals were sacrificed and blood collected for determination of biochemical parameters using standard procedures and techniques.
Exposure to Cd produced greater increases in the liver function parameters. However treatment with SM significantly (p<0.05) reduced ALT in animals treated after exposure, AST and bilirubin in those treated before exposure and significant (p<0.05) increased serum albumin in animals treated before exposure to Cd. The altered renal function parameters and total serum cholesterol were restored to near normal values following treatment with SM.
It may be concluded that concurrent intake of garlic, ginger and nutmeg at culinary dose in the diet has both therapeutic and prophylactic effect at mitigating Cd toxicity and reaffirms the safely of spices combinations as being currently practiced.
确定含有大蒜、生姜和肉豆蔻的混合香料对镉暴露大鼠肝脏和肾脏的潜在保护作用。
选用体重120 - 180克的雄性白化大鼠（n = 30），分为五组（1 - 5组），每组6只大鼠进行研究。第1组（NC）大鼠口服蒸馏水（1毫升），持续4周，作为阴性对照；第2组（PC）大鼠口服低剂量（LD）镉（25毫克/千克体重），持续4周，作为阳性对照。第3组（TBE）大鼠先用混合香料（SM）治疗；口服300毫克/千克体重，持续2周，然后给予低剂量镉，持续4周。第4组（CET）大鼠同时给予低剂量镉和混合香料，持续4周，第5组（TAE）大鼠给予低剂量镉，持续4周，然后用混合香料治疗2周。整个实验持续42天，之后处死动物并采集血液，使用标准程序和技术测定生化参数。
镉暴露使肝功能参数大幅升高。然而，混合香料治疗显著（p<0.05）降低了暴露后治疗动物的谷丙转氨酶（ALT）、暴露前治疗动物的谷草转氨酶（AST）和胆红素，且显著（p<0.05）提高了镉暴露前治疗动物的血清白蛋白。混合香料治疗后，肾功能参数改变和总血清胆固醇恢复到接近正常水平。
可以得出结论，饮食中按烹饪剂量同时摄入大蒜、生姜和肉豆蔻在减轻镉毒性方面具有治疗和预防作用，并重申了目前使用的香料组合的安全性。
