Ibiam Ama Udu, Ugwuja Emmanuel Ike, Ejeogo Christ, Ugwu Okechukwu
Department of Biochemistry, Faculty of Biological Sciences, Ebonyi State University, PMB 053, Abakaliki, Nigeria.
Adv Pharm Bull. 2013;3(2):309-13. doi: 10.5681/apb.2013.050. Epub 2013 Aug 20.
Hepatoprotective potentials of Jussiaea nervosa leaf extract against Cadmium-induced hepatotoxicity were investigated.
Forty albino rats were randomly assigned into groups A-G with 4 rats in each of the groups A-F. Group A served as control and were given feed only while rats in groups B-F were orally exposed to varying concentrations of cadmium for six weeks. Effects of cadmium were most significant at 12 mg/Kg body weight (BW), and this dose was used for subsequent test involving oral administration of Jussiaea nervosa leaf extracts. In this segment, group G (n= 16) was sub-divided into four: G1-G4, with each sub-group containing four rats. Rats in sub-group G1 were given cadmium and feed only and served as positive control. Rats in sub-groups G2, G3, and G4 were given cadmium and 20, 50 and 100g/kg BW of Jussiaea nervosa extract, respectively, for six weeks. Blood and liver were analysed using standard laboratory techniques and methods.
Liver function parameters (ALT, AST, ALP, bilirubin) were significantly (p<0.05) elevated in exposed rats in comparison to the controls, except for total protein and albumin, which were significantly decreased. Histopathological assessment reveals renal pathology in exposed rats in sharp contrast with the controls. Jussiaea nervosa extract however lowered the values of liver function parameters with 100mg/Kg BW dose producing the highest ameliorative effects. Similarly, the serum albumin and total protein significantly (p<0.05) improved with normal liver architecture.
The results show the hepatoprotective potentials of Jussiaea nervosa extract against Cd toxicity.
研究水蓑衣叶提取物对镉诱导的肝毒性的肝脏保护潜力。
将40只白化大鼠随机分为A - G组，A - F组每组4只。A组作为对照组，仅给予饲料，而B - F组大鼠口服不同浓度的镉，持续六周。镉在12mg/Kg体重时的影响最为显著，该剂量用于后续涉及口服水蓑衣叶提取物的试验。在这一阶段，G组（n = 16）分为四个亚组：G1 - G4，每个亚组包含4只大鼠。G1亚组的大鼠仅给予镉和饲料，作为阳性对照。G2、G3和G4亚组的大鼠分别给予镉以及20、50和100g/kg体重的水蓑衣提取物，持续六周。使用标准实验室技术和方法对血液和肝脏进行分析。
与对照组相比，暴露组大鼠的肝功能参数（ALT、AST、ALP、胆红素）显著（p<0.05）升高，但总蛋白和白蛋白显著降低。组织病理学评估显示，暴露组大鼠出现肾脏病变，与对照组形成鲜明对比。然而，水蓑衣提取物降低了肝功能参数的值，其中100mg/Kg体重剂量的改善效果最为显著。同样，血清白蛋白和总蛋白显著（p<0.05）改善，肝脏结构正常。
结果表明水蓑衣提取物对镉毒性具有肝脏保护潜力。
