Badenes Sara M, Fernandes Tiago G, Rodrigues Carlos A V, Diogo Maria Margarida, Cabral Joaquim M S
Department of Bioengineering, and Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Portugal.
Department of Bioengineering, and Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Portugal.
J Biotechnol. 2016 Sep 20;234:71-82. doi: 10.1016/j.jbiotec.2016.07.023. Epub 2016 Jul 29.
Human pluripotent stem cells (hPSC) have attracted a great attention as an unlimited source of cells for cell therapies and other in vitro biomedical applications such as drug screening, toxicology assays and disease modeling. The implementation of scalable culture platforms for the large-scale production of hPSC and their derivatives is mandatory to fulfill the requirement of obtaining large numbers of cells for these applications. Microcarrier technology has been emerging as an effective approach for the large scale ex vivo hPSC expansion and differentiation. This review presents recent achievements in hPSC microcarrier-based culture systems and discusses the crucial aspects that influence the performance of these culture platforms. Recent progress includes addressing chemically-defined culture conditions for manufacturing of hPSC and their derivatives, with the development of xeno-free media and microcarrier coatings to meet good manufacturing practice (GMP) quality requirements. Finally, examples of integrated platforms including hPSC expansion and directed differentiation to specific lineages are also presented in this review.
人类多能干细胞（hPSC）作为细胞治疗及其他体外生物医学应用（如药物筛选、毒理学检测和疾病建模）的无限细胞来源，已引起了广泛关注。为满足这些应用对大量细胞的需求，必须实施可扩展的培养平台来大规模生产hPSC及其衍生物。微载体技术已成为大规模体外hPSC扩增和分化的有效方法。本文综述了基于hPSC微载体培养系统的最新成果，并讨论了影响这些培养平台性能的关键因素。最近的进展包括为hPSC及其衍生物的生产确定化学定义的培养条件，开发无动物源培养基和微载体涂层以满足良好生产规范（GMP）质量要求。最后，本文还介绍了包括hPSC扩增和定向分化为特定谱系的综合平台实例。
