Chen Yuan, Zou Ting, McCormick Sheila
Plant Gene Expression Center, United States Department of Agriculture-Agricultural Research Service, and Department of Plant and Microbial Biology, University of California Berkeley, Albany, California 94710.
Plant Gene Expression Center, United States Department of Agriculture-Agricultural Research Service, and Department of Plant and Microbial Biology, University of California Berkeley, Albany, California 94710
Plant Physiol. 2016 Sep;172(1):244-53. doi: 10.1104/pp.16.00774. Epub 2016 Aug 1.
S-Adenosylmethionine is widely used in a variety of biological reactions and participates in the methionine (Met) metabolic pathway. In Arabidopsis (Arabidopsis thaliana), one of the four S-adenosylmethionine synthetase genes, METHIONINE ADENOSYLTRANSFERASE3 (MAT3), is highly expressed in pollen. Here, we show that mat3 mutants have impaired pollen tube growth and reduced seed set. Metabolomics analyses confirmed that mat3 pollen and pollen tubes overaccumulate Met and that mat3 pollen has several metabolite profiles, such as those of polyamine biosynthesis, which are different from those of the wild type. Additionally, we show that disruption of Met metabolism in mat3 pollen affected transfer RNA and histone methylation levels. Thus, our results suggest a connection between metabolism and epigenetics.
S-腺苷甲硫氨酸广泛应用于各种生物反应,并参与甲硫氨酸(Met)代谢途径。在拟南芥中,四个S-腺苷甲硫氨酸合成酶基因之一的甲硫氨酸腺苷转移酶3(MAT3)在花粉中高度表达。在此,我们表明mat3突变体的花粉管生长受损且结实率降低。代谢组学分析证实,mat3花粉和花粉管中Met过度积累,并且mat3花粉具有几种代谢物谱,例如多胺生物合成的代谢物谱,与野生型不同。此外,我们表明mat3花粉中Met代谢的破坏影响了转运RNA和组蛋白甲基化水平。因此,我们的结果表明了代谢与表观遗传学之间的联系。