Rubio C A, Lindholm J, Rodensjö M
Department of Pathology, Karolinska Institute, Stockholm, Sweden.
Pathol Res Pract. 1989 May;184(5):525-8. doi: 10.1016/S0344-0338(89)80146-3.
A gastrectomy specimen housing an adenocarcinoma of intestinal type was divided into 60 blocks. Sections were stained with Alcian blue pH 2.5 (to detect foreign mucins). Alcian blue fields were quantitated with a MOP 30 connected to a computer. The mucosa analysed in the 60 sections measured 3788.3 mm2 and that occupied by Alcian blue stained goblet cells 300.9 mm2 (or 7.9% of the total mucosal area). The number of Alcian blue positive fields was significantly larger in the intermediate zone followed by the antral zone, the lowest value was found in the corpus. However, the gastric mucosa with Alcian blue positive cells was larger in the antrum than in the intermediate zone due to the fact that the area of the fields with Alcian blue positive cells was significantly larger in the antrum than in the intermediate zone. The size and number of Alcian blue positive fields were significantly larger along the greater than along the smaller curvature. All measurements were done by a trained technician. The method (using histochemistry, morphometry and skilled technical assistance) will be applied to study the distribution of intestinal metaplasia in gastrectomy specimens from populations with disparate incidences of gastric carcinoma.
一个包含肠型腺癌的胃切除标本被切成60个组织块。切片用pH 2.5的阿尔辛蓝染色（以检测外源性黏蛋白）。用连接计算机的MOP 30对阿尔辛蓝染色区域进行定量分析。60个切片中分析的黏膜面积为3788.3平方毫米，被阿尔辛蓝染色的杯状细胞占据的面积为300.9平方毫米（占黏膜总面积的7.9%）。阿尔辛蓝阳性区域的数量在中间区显著多于胃窦区，胃体部最低。然而，由于胃窦区阿尔辛蓝阳性细胞区域的面积显著大于中间区，所以胃窦部有阿尔辛蓝阳性细胞的胃黏膜面积大于中间区。阿尔辛蓝阳性区域的大小和数量在大弯侧显著大于小弯侧。所有测量均由一名训练有素的技术人员完成。该方法（使用组织化学、形态计量学和熟练的技术协助）将用于研究来自胃癌发病率不同人群的胃切除标本中肠化生的分布情况。
