Türedi Sibel, Yuluğ Esin, Alver Ahmet, Kutlu Ömer, Kahraman Cemil
Karadeniz Technical University, Faculty of Medicine, Department of Histology and Embryology, 61080 Trabzon, Turkey.
Karadeniz Technical University, Faculty of Medicine, Department of Medical Biochemistry, 61080 Trabzon, Turkey.
Exp Toxicol Pathol. 2015 Mar;67(3):229-35. doi: 10.1016/j.etp.2014.12.002. Epub 2014 Dec 29.
The purpose of this study was to evaluate the likely protective effect of resveratrol (RES) on doxorubicin (DOX) induced testicular damage. Rats were divided into five groups: control, RES, dimethyl sulfoxide (DMSO), DOX and DOX+RES. At the end of treatment, the rats were sacrificed. Plasma testosterone levels, oxidative status, epididymal sperm parameters and testicular apoptosis were evaluated. MDA levels, GP-x and GSH activities were higher in the DOX group than in the control group. MDA levels were lower in the DOX+RES group than in the DOX group. The DOX group exhibited a significant decrease in plasma testosterone levels, sperm concentration and motility, and a significant increase in abnormal sperm rate and TUNEL (+) cells in the testis. A significant increase was observed in plasma testosterone levels and sperm concentration and motility, and a significant decrease in the abnormal sperm rate and TUNEL (+) cells in the DOX+RES group compared to the DOX group. A marked improvement in severe degenerative alterations in the germinative epithelium was also observed following treatment with RES. In conclusion, RES makes a positive contribution to fertility by exhibiting anti-apoptotic and antiperoxidative effects against DOX-induced testicular damage.
本研究的目的是评估白藜芦醇（RES）对阿霉素（DOX）诱导的睾丸损伤可能具有的保护作用。将大鼠分为五组：对照组、RES组、二甲基亚砜（DMSO）组、DOX组和DOX + RES组。治疗结束时，处死大鼠。评估血浆睾酮水平、氧化状态、附睾精子参数和睾丸细胞凋亡情况。DOX组的丙二醛（MDA）水平、谷胱甘肽过氧化物酶（GP-x）和谷胱甘肽（GSH）活性高于对照组。DOX + RES组的MDA水平低于DOX组。DOX组的血浆睾酮水平、精子浓度和活力显著降低，睾丸中异常精子率和TUNEL（+）细胞显著增加。与DOX组相比，DOX + RES组的血浆睾酮水平、精子浓度和活力显著升高，异常精子率和TUNEL（+）细胞显著降低。用RES治疗后，还观察到生精上皮严重退行性改变有明显改善。总之，RES通过对DOX诱导的睾丸损伤表现出抗凋亡和抗过氧化作用，对生育能力有积极贡献。
