Tijsma Aloys, Thibaut Hendrik Jan, Franco David, Dallmeier Kai, Neyts Johan
Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, University of Leuven, Leuven, Belgium.
Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, University of Leuven, Leuven, Belgium.
Antiviral Res. 2016 Sep;133:106-9. doi: 10.1016/j.antiviral.2016.07.023. Epub 2016 Jul 30.
It has been generally accepted that hydantoin [5-(3,4-dichlorophenyl)methylhydantoin] exerts its anti-enterovirus activity by solely inhibiting viral assembly. However, we here show that hydantoin inhibits enteroviral RNA synthesis as well as subgenomic replication in a dose-dependent manner. We demonstrate that inhibition of RNA synthesis is the predominant mechanism of action at relatively high concentrations of hydantoin. However, at lower concentrations inhibition of viral morphogenesis is the main mechanism of action. Thus, hydantoin inhibits enteroviral replication by two distinct mechanisms.
人们普遍认为，乙内酰脲[5-(3,4-二氯苯基)甲基乙内酰脲]仅通过抑制病毒组装发挥其抗肠道病毒活性。然而，我们在此表明，乙内酰脲以剂量依赖的方式抑制肠道病毒RNA合成以及亚基因组复制。我们证明，在相对高浓度的乙内酰脲下，RNA合成的抑制是主要作用机制。然而，在较低浓度下，病毒形态发生的抑制是主要作用机制。因此，乙内酰脲通过两种不同机制抑制肠道病毒复制。
