Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Department of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands.
Antiviral Res. 2015 May;117:110-4. doi: 10.1016/j.antiviral.2015.02.013. Epub 2015 Mar 6.
Enteroviruses, e.g., polio-, coxsackie- and rhinoviruses, constitute a large genus within the Picornaviridae family of positive-strand RNA viruses and include many important pathogens linked to a variety of acute and chronic diseases. Despite their huge medical and economic impact, no approved antiviral therapy is yet available. Recently, the oxysterol-binding protein (OSBP) was implicated as a host factor for enterovirus replication. Here, we investigated the antiviral activity of the natural compound OSW-1, a ligand of OSBP that is under investigation as an anti-cancer drug. OSW-1 potently inhibited the replication of all enteroviruses tested, with IC50 values in the low nanomolar range, acted at the genome replication stage and was effective in all tested cell types of three different species. Importantly, OSBP overexpression rescued viral replication, demonstrating that the antiviral effect of OSW-1 is due to targeting OSBP. Together, we here report the anti-enterovirus activity of the natural anti-cancer compound OSW-1.
肠道病毒，例如脊髓灰质炎病毒、柯萨奇病毒和鼻病毒，构成了正链 RNA 病毒小核糖核酸病毒科中的一个大属，包含许多与多种急性和慢性疾病相关的重要病原体。尽管它们具有巨大的医学和经济影响，但目前尚无批准的抗病毒疗法。最近，氧化固醇结合蛋白（OSBP）被认为是肠道病毒复制的宿主因子。在这里，我们研究了天然化合物 OSW-1 的抗病毒活性，OSW-1 是 OSBP 的配体，作为一种抗癌药物正在研究中。OSW-1 能够强烈抑制所有测试的肠道病毒的复制，其半数抑制浓度（IC50）值处于纳摩尔低水平，作用于基因组复制阶段，在三种不同物种的所有测试细胞类型中均有效。重要的是，OSBP 的过表达挽救了病毒复制，表明 OSW-1 的抗病毒作用是由于靶向 OSBP。总之，我们在这里报告了天然抗癌化合物 OSW-1 的抗肠道病毒活性。
