Li Sun, Liu Xiao-Lin, Zhou Xie-Lai, Jiang Su-Jun, Yuan Hong
Medical School, Hangzhou Normal University, No16, Xue Lin street, Xia Sha, Hangzhou, 310036, Zhejiang, China.
Interdiscip Toxicol. 2015 Dec;8(4):155-8. doi: 10.1515/intox-2015-0024.
The toxic metal lead is a widespread environmental polutant that can adversely affect human health. However, the underlying mechanisms of lead-induced toxicity are still largely unknown. The mechanism of lead toxicity was presumed to involve cross reaction between Pb(2+) and Ca(2+) with calmodulin dependent systems. The aim of the present study was thus to identify differential expression of calmodulin-related genes in the spleen of lead-exposed mice. We performed microarray analysis to identify differentially expressed genes. RNAs from spleen tissue of lead exposed animals (n=6) and controls (n=6) were converted to labeled cRNA and hybridized to Illumina mouse WG-6_v2_Bead Chip. Expression profiles were analyzed using Illumina BeadStudio Application. Real-time RT-PCR was conducted to validate the microarray data. By microarray analysis 5 calmodulin-related genes (MAP2K6, CAMKK2, CXCR4, PHKA2, MYLK) were found to be differently expressed in lead exposed compared with control mice (p<0.05). The results of Real-time RT-PCR showed that MAP2K6 and CAMKK2 were up-regulated and CXCR4 was down-regulated in lead exposure, but there were no significant differences in PHKA2 and MYLK expression between the lead exposed and control group. These results show that lead exposure produced significant changes in expression of a variety of genes in the spleen and can affect calmodulin-related gene expression.
有毒金属铅是一种广泛存在的环境污染物，会对人类健康产生不利影响。然而，铅诱导毒性的潜在机制在很大程度上仍不清楚。铅毒性的机制被推测涉及Pb(2+)与Ca(2+)在钙调蛋白依赖性系统中的交叉反应。因此，本研究的目的是确定铅暴露小鼠脾脏中钙调蛋白相关基因的差异表达。我们进行了微阵列分析以鉴定差异表达的基因。将来自铅暴露动物（n=6）和对照组（n=6）脾脏组织的RNA转化为标记的cRNA，并与Illumina小鼠WG-6_v2_Bead芯片杂交。使用Illumina BeadStudio应用程序分析表达谱。进行实时RT-PCR以验证微阵列数据。通过微阵列分析，发现与对照小鼠相比，5个钙调蛋白相关基因（MAP2K6、CAMKK2、CXCR4、PHKA2、MYLK）在铅暴露组中表达存在差异（p<0.05）。实时RT-PCR结果显示，铅暴露时MAP2K6和CAMKK2上调，CXCR4下调，但铅暴露组和对照组之间PHKA2和MYLK的表达没有显著差异。这些结果表明，铅暴露会使脾脏中多种基因的表达产生显著变化，并可能影响钙调蛋白相关基因的表达。
