Dyson Zoe A, Brown Teagan L, Farrar Ben, Doyle Stephen R, Tucci Joseph, Seviour Robert J, Petrovski Steve
La Trobe Institute of Molecular Sciences, Bendigo, VIC, Australia.
Department of Animal, Plant & Soil Sciences, Latrobe University, Bundoora, VIC, Australia.
PLoS One. 2016 Aug 3;11(8):e0159957. doi: 10.1371/journal.pone.0159957. eCollection 2016.
Little is known about the prevalence, functionality and ecological roles of temperate phages for members of the mycolic acid producing bacteria, the Mycolata. While many lytic phages infective for these organisms have been isolated, and assessed for their suitability for use as biological control agents of activated sludge foaming, no studies have investigated how temperate phages might be induced for this purpose. Bioinformatic analysis using the PHAge Search Tool (PHAST) on Mycolata whole genome sequence data in GenBank for members of the genera Gordonia, Mycobacterium, Nocardia, Rhodococcus, and Tsukamurella revealed 83% contained putative prophage DNA sequences. Subsequent prophage inductions using mitomycin C were conducted on 17 Mycolata strains. This led to the isolation and genome characterization of three novel Caudovirales temperate phages, namely GAL1, GMA1, and TPA4, induced from Gordonia alkanivorans, Gordonia malaquae, and Tsukamurella paurometabola, respectively. All possessed highly distinctive dsDNA genome sequences.
关于温和噬菌体在产分枝菌酸细菌（分枝杆菌纲）中的流行情况、功能及生态作用，人们了解甚少。虽然已分离出许多可感染这些生物体的裂解性噬菌体，并评估了它们作为活性污泥泡沫生物控制剂的适用性，但尚无研究调查如何为此目的诱导温和噬菌体。利用PHAge搜索工具（PHAST）对GenBank中戈登氏菌属、分枝杆菌属、诺卡氏菌属、红球菌属和冢村氏菌属成员的分枝杆菌纲全基因组序列数据进行生物信息学分析，结果显示83%含有推定的前噬菌体DNA序列。随后对17株分枝杆菌纲菌株使用丝裂霉素C进行前噬菌体诱导。这导致分别从食烷戈登氏菌、马来亚戈登氏菌和迟缓冢村氏菌中诱导分离出三种新型有尾噬菌体目温和噬菌体，即GAL1、GMA1和TPA4，并对其基因组进行了表征。它们均拥有高度独特的双链DNA基因组序列。
