术前臭氧治疗对手术伤口愈合的急性影响。

The acute effects of preoperative ozone theraphy on surgical wound healing.

作者信息

Sahin Hasan, Simsek Tuncer, Turkon Hakan, Kalkan Yıldıray, Ozkul Faruk, Ozkan M Turgut Alper, Erbas Mesut, Altinisik Ugur, Demiraran Yavuz

机构信息

Assistant Professor, Department of Anesthesiology and Reanimation, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey. Conception and design of the study; acquisition, analyzis and interpretation of data; manuscript writing; critical revision; final approved.

Assistant Professor, Department of Biochemistry, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey. of data. Biochemical examinations, final approved.

出版信息

Acta Cir Bras. 2016 Jul;31(7):472-8. doi: 10.1590/S0102-865020160070000007.

DOI:10.1590/S0102-865020160070000007

PMID:27487282

Abstract

PURPOSE

To investigate the effects of preoperative rectal ozone insufflation on surgical wound healing over the proinflammatory cytokines and histopathological changes.

METHODS

Twenty one rabbits were divided into 3 groups. Sham, surgical wound, and ozone applied (6 sessions, every other day 70 µg/mL in 12 mL O2-O3 mixture rectally) surgical wound groups were created. TNF-alpha and IL-6 levels from all rabbits were studied at the basal, 24th hour, and 72nd hour. The histopathological examination was done by removing the surgical scar tissue at the end of 72nd hour.

RESULTS

TNF-alfa and IL-6 levels were significantly lower compared to the control group, in the rabbits treated with ozone. The increase in angiogenesis, the decrease in the number of inflammatory cells, epidermal and dermal regeneration, better collagen deposition, and increased keratinisation in stratum corneum were observed in the histopathological examination. It was determined that the wound healing noticeably accelerated in the ozone group.

CONCLUSION

Preoperative rectal ozone insufflation had a positive effect on surgical wound healing in acute period.

摘要

目的

探讨术前直肠注入臭氧对手术伤口愈合的影响，以及促炎细胞因子和组织病理学变化。

方法

将21只兔子分为3组。分别创建假手术组、手术伤口组和臭氧应用组（6次，每隔一天直肠注入12 mL氧气 - 臭氧混合物中的70 µg/mL臭氧）。在基础状态、第24小时和第72小时研究所有兔子的肿瘤坏死因子 - α（TNF - α）和白细胞介素 - 6（IL - 6）水平。在第72小时结束时切除手术瘢痕组织进行组织病理学检查。