臭氧通过下调白细胞介素-8、10 水平和诱导成纤维细胞生长因子受体表达来改善 2 型糖尿病患者的伤口愈合。
Ozone improved the wound healing in type 2 diabetics via down-regulation of IL- 8, 10 and induction of FGFR expression.
机构信息
Department of General Surgery named after Professor M. I. Gulman, Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russian Federation.
Department of General Surgery named after Professor M. I. Gulman, Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russian Federation; Center for collective use «Molecular & cell technologies», Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russian Federation .
出版信息
Acta Biomed. 2022 May 11;93(2):e2022060. doi: 10.23750/abm.v93i2.12291.
BACKGROUND AND AIM
We aimed to investigate the effect of ozonated autohaemotherapy (OA) on the wound healing, serum values of interleukin (IL) - 6, 8, 10, tumor necrosis factor-alpha (TNF-α), basic fibroblast growth factor (bFGF) and local expression of fibroblast growth factor receptors (FGFR) in type 2 diabetics with the acute soft-tissue infections.
METHODS
Patients in the first cohort (n-30) received a basic comprehensive treatment (BCT-group), and the second (n=28) also received OA (OA-group). Blood samples for ELISA and tissue specimens for the immunohistochemical examinations were collected at admission (day 0) and at the 9th day of inpatient treatment.
RESULTS
The additional using of OA has accelerated the timing of a single and the complete wound granulation and the timing to marginal epithelization, compared with the results of the standard treatment. The use of OA has significantly reduced the production of IL-8, 10 at 9th day. OA-group patients were characterized by consistently high levels of bFGF production in contrast to the BCT-group, where the decreasing in the serum bFGF level was observed. The maximum number of bFGFR - immunopositive labels was observed in OA-group out to 9th day (319,45 (249,90-348,43) versus baseline 192,65 (171,93-207,72), versus BCT-group 123,30 (105,23- 141,10), p<0,001).
CONCLUSIONS
Application of OA in the complex treatment of the acute soft-tissue infections in diabetics makes it possible to achieve the significant reductions in the duration of the wound inflammation and regeneration phases by eliminating of overproduction of IL- 8, 10 and induction of expression of bFGF and its receptors.
背景与目的
我们旨在研究臭氧自体血疗法（OA）对伴有急性软组织感染的 2 型糖尿病患者的伤口愈合、血清白细胞介素（IL）-6、8、10、肿瘤坏死因子-α（TNF-α）、碱性成纤维细胞生长因子（bFGF）和局部成纤维细胞生长因子受体（FGFR）表达的影响。
方法
第一组患者（n=30）接受基本综合治疗（BCT 组），第二组（n=28）还接受 OA（OA 组）。在入院时（第 0 天）和住院治疗第 9 天采集 ELISA 血液样本和免疫组织化学检查的组织标本。
结果
与标准治疗相比，OA 的额外使用加速了单一和完全肉芽形成的时间以及边缘上皮化的时间。OA 组在第 9 天时 IL-8、10 的产生明显减少。与 BCT 组相比，OA 组患者的 bFGF 产生持续高水平，而 BCT 组则观察到血清 bFGF 水平下降。到第 9 天，OA 组观察到 bFGFR 免疫阳性标记的最大数量（319.45（249.90-348.43）与基线 192.65（171.93-207.72）相比，与 BCT 组 123.30（105.23-141.10）相比，p<0.001）。
结论
在糖尿病患者急性软组织感染的综合治疗中应用 OA 可以通过消除过度产生的 IL-8、10 和诱导 bFGF 及其受体的表达，显著缩短伤口炎症和再生阶段的持续时间。
Zotero 插件