Zhang Tianyuan, Deng Xiaoyu, Yu Ying, Zhang Mengyue, Zhang Yixuan
School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
Int J Syst Evol Microbiol. 2016 Nov;66(11):4377-4381. doi: 10.1099/ijsem.0.001359. Epub 2016 Aug 2.
An endophytic strain (designated as SYPF 7195T) was isolated from a branch of a ginkgo tree in Liaoning province of China. Strain SYPF 7195T was characterized by its grey to greyish-green aerial mycelium, velvety to floccose surface and swelling near the septa. Phylogenetic analyses, which were inferred from the internal transcribed spacer (ITS) and partial sequences of the LSU and SSU of the rDNA and translation elongation factor 1-alpha (TEF1), showed that strain SYPF 7195T belonged to the genus Pseudochaetosphaeronema, and was distinct from all other species with high bootstrap-supported values (92 %). Strain SYPF 7195T constitutes a separate evolutionary clade with Pseudochaetosphaeronema larense and Pseudochaetosphaeronema martinelli, with P. martinelli as its closest phylogenetic neighbour. The nucleotide differences between strain SYPF 7195T and P. martinelli were 71 substitutions in the ITS region. Strain SYPF 7195Tcould also be distinguished from P. martinelli by a number of physiological characteristics. Combined with morphology and molecular analyses, strain SYPF 7195T merits recognition as a representative of a novel species of the genus Pseudochaetosphaeronema, for which the name Pseudochaetosphaeronemaginkgonis sp. nov. is proposed. The type strain is CBS 140953T (=CGMCC 3.17865T=SYPF 7195T). The Mycobank number is MB 816567.
从中国辽宁省的一棵银杏树的树枝中分离出一株内生菌株（命名为SYPF 7195T）。菌株SYPF 7195T的特征在于其气生菌丝体为灰色至灰绿色，表面呈天鹅绒状至絮状，且在隔膜附近肿胀。基于核糖体DNA的内转录间隔区（ITS）、大亚基（LSU）和小亚基（SSU）的部分序列以及翻译延伸因子1-α（TEF1）进行的系统发育分析表明，菌株SYPF 7195T属于拟毛球腔菌属，与所有其他物种不同，具有较高的自展支持值（92%）。菌株SYPF 7195T与拉氏拟毛球腔菌和马丁内利拟毛球腔菌构成一个单独的进化分支，其中马丁内利拟毛球腔菌是其最接近的系统发育邻居。菌株SYPF 7195T与马丁内利拟毛球腔菌在ITS区域的核苷酸差异为71个替换。菌株SYPF 7195T在一些生理特征上也可与马丁内利拟毛球腔菌区分开来。结合形态学和分子分析，菌株SYPF 7195T值得被认可为拟毛球腔菌属一个新物种的代表，为此提出新物种名称拟毛球腔菌银杏种（Pseudochaetosphaeronema ginkgonis sp. nov.）。模式菌株为CBS 140953T（=CGMCC 3.17865T=SYPF 7195T）。国际真菌命名数据库编号为MB 816567。
