Zou Kai, Liu Xueduan, Hu Qi, Zhang Du, Fu Shaodong, Zhang Shuangfei, Huang Haonan, Lei Fangying, Zhang Guoqing, Miao Bo, Meng Delong, Jiang Luhua, Liu Hongwei, Yin Huaqun, Liang Yili
School of Minerals Processing and Bioengineering, Central South University, Changsha, China.
Key Laboratory of Biometallurgy of Ministry of Education, Changsha, China.
Front Plant Sci. 2021 Jul 9;12:704985. doi: 10.3389/fpls.2021.704985. eCollection 2021.
is a pharmaceutical resource for terpenes and flavonoids. However, few insights discussed endophytes' role in , and whether genetic exchange happens between and endophytes remains unclear. Herein, functional gene profiles and repetitive sequences were analyzed to focus on these issues. A total of 25 endophyte strains were isolated from the root and distributed in 16 genera of 6 phyla. Significant morphological diversities lead to the diversity in the COG functional classification. KEGG mapping revealed that endophytic bacteria and fungi potentially synthesize chalcone, while endophytic fungi might also promote flavonoid derivatization. Both bacteria and fungi may facilitate the lignin synthesis. sp. Gbtc_1 exhibited the feasibility of regulating alcohols to lignans. Although and the endophytes have not observed the critical levopimaradiene synthase in ginkgolides synthesis, the upstream pathways of terpenoid precursors are likely intact. The MVK genes in may have alternative non-homologous copies or be compensated by endophytes in long-term symbiosis. sp. Gbtc_1 became the only bacteria to harbor both MEP and MVA pathways. Endophytes may perform the mutual transformation of IPP and DMAPP in the root. and bacteria may lead to the synthesis and derivatization of the carotenoid pathway. The isoquinoline alkaloid biosynthesis seemed lost in the root community, but L-dopa is more probably converted into dopamine as an essential signal-transduction substance. So, endophytes may participate in the secondary metabolism of the in a shared or complementary manner. Moreover, a few endophytic sequences predicted as and superfamilies exhibited extremely high similarity to those of . CDSs in such endophytic LTR-RT sequences were also highly homologous to one CDS. Therefore, LTR-RTs may be a rare unit flowing between the host and endophytes to exchange genetic information. Collectively, this research effectively expanded the insight on the symbiotic relationship between the host and the endophytes in the root.
是萜类化合物和黄酮类化合物的药用资源。然而，很少有见解讨论内生菌在……中的作用，并且……与内生菌之间是否发生基因交换仍不清楚。在此，分析了功能基因图谱和重复序列以关注这些问题。从……根部分离出总共25株内生菌菌株，分布在6个门的16个属中。显著的形态多样性导致了COG功能分类的多样性。KEGG图谱显示内生细菌和真菌可能合成查耳酮，而内生真菌也可能促进黄酮类化合物的衍生化。细菌和真菌都可能促进木质素的合成。……Gbtc_1菌株展示了将醇类调节为木脂素的可行性。尽管……和内生菌在银杏内酯合成中未观察到关键的左旋海松二烯合酶，但萜类前体的上游途径可能是完整的。……中的MVK基因可能有替代的非同源拷贝，或者在长期共生中由内生菌补偿。……Gbtc_1菌株成为唯一同时拥有MEP和MVA途径的细菌。内生菌可能在根中进行IPP和DMAPP的相互转化。……和细菌可能导致类胡萝卜素途径的合成和衍生化。异喹啉生物碱的生物合成似乎在……根群落中丢失，但L - 多巴更有可能转化为多巴胺作为一种重要的信号转导物质。因此，内生菌可能以共享或互补的方式参与……的次生代谢。此外，一些预测为……和……超家族的内生菌序列与……的序列表现出极高的相似性。此类内生菌LTR - RT序列中的CDS也与一个……CDS高度同源。因此，LTR - RTs可能是在……宿主和内生菌之间流动以交换遗传信息的稀有单元。总体而言，这项研究有效地扩展了对……宿主与根中内生菌共生关系的认识。
