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各种碳水化合物生物制氢的化学计量学评估。

Stoichiometry evaluation of biohydrogen production from various carbohydrates.

作者信息

Amin Mohammad Mehdi, Bina Bijan, Taheri Ensiyeh, Fatehizadeh Ali, Ghasemian Mohammad

机构信息

Environment Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.

Department of Environmental Health Engineering, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran.

出版信息

Environ Sci Pollut Res Int. 2016 Oct;23(20):20915-20921. doi: 10.1007/s11356-016-7244-6. Epub 2016 Aug 3.

DOI:10.1007/s11356-016-7244-6
PMID:27488706
Abstract

In this paper, biochemical hydrogen potential (BHP) tests were conducted to investigate H production from different substrate with acid-treated anaerobic digested sludge at the mesophilic range. The sludge was collected from an anaerobic digester and was subjected to sulfuric acid pretreatments at pH 3 for 24 h. The effects of substrate type (glucose, fructose, and sucrose as carbon source) were investigated in batch experiments. Results showed that substrate degradation rate for all of the substrates was up 95 % and the electron equivalent balance showed good closure for glucose and sucrose. Batch experiments showed that the maximum molar hydrogen yield with glucose, fructose, and sucrose was 3.27, 3.16, and 6.46 mol H/mol of substrate. The maximum cumulative biohydrogen production was 1552, 1487, and 1366 mL and maximum hydrogen production rate was 308, 279, and 275 mL/h for glucose, sucrose, and fructose, respectively. The experimental results suggest that the formation of hydrogen associates with the main aqueous products, i.e., acetate butyrate.

摘要

本文进行了生化氢潜力(BHP)测试,以研究在中温范围内经酸处理的厌氧消化污泥利用不同底物产氢的情况。污泥取自一个厌氧消化池,并在pH值为3的条件下用硫酸预处理24小时。在批次实验中研究了底物类型(葡萄糖、果糖和蔗糖作为碳源)的影响。结果表明,所有底物的降解率均高达95%,且电子当量平衡显示葡萄糖和蔗糖的平衡良好。批次实验表明,以葡萄糖、果糖和蔗糖为底物时,最大摩尔产氢量分别为3.27、3.16和6.46摩尔氢气/摩尔底物。葡萄糖、蔗糖和果糖的最大累积生物产氢量分别为1552、1487和1366毫升,最大产氢速率分别为308、279和275毫升/小时。实验结果表明,氢气的形成与主要的水性产物即乙酸盐和丁酸盐有关。

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本文引用的文献

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Hydrogen production from sugar beet juice using an integrated biohydrogen process of dark fermentation and microbial electrolysis cell.利用黑暗发酵和微生物电解池集成生物制氢工艺从糖甜菜汁中生产氢气。
Bioresour Technol. 2015 Dec;198:223-30. doi: 10.1016/j.biortech.2015.08.048. Epub 2015 Sep 2.
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Effective conversion of maize straw wastes into bio-hydrogen by two-stage process integrating H2 fermentation and MECs.通过结合氢气发酵和微生物电解池的两阶段工艺将玉米秸秆废料有效转化为生物氢气。
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One-carbon substrate-based biohydrogen production: microbes, mechanism, and productivity.
乙酸-丁酸与乙酸-乙醇生物制氢代谢途径的比较
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An electron-flow model can predict complex redox reactions in mixed-culture fermentative bioH2: microbial ecology evidence.电子流模型可以预测混合培养发酵生物制氢中的复杂氧化还原反应:微生物生态学证据。
Biotechnol Bioeng. 2009 Nov 1;104(4):687-97. doi: 10.1002/bit.22442.
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Evaluation of metabolism using stoichiometry in fermentative biohydrogen.利用化学计量学评估发酵生物制氢中的代谢情况。
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Evaluation of methods for preparing hydrogen-producing seed inocula under thermophilic condition by process performance and microbial community analysis.通过工艺性能和微生物群落分析评估嗜热条件下产氢种子接种物的制备方法
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