Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Front Endocrinol (Lausanne). 2023 Jul 14;14:1126572. doi: 10.3389/fendo.2023.1126572. eCollection 2023.
This study was designed to explore the composition of the intestinal microbiota and its longitudinal variation between the second trimester (T2) and the third trimester (T3) in women with gestational diabetes mellitus (GDM) and pregnant women with normal glucose tolerance.
This observational study was conducted at Peking Union Medical College Hospital (PUMCH). Women with GDM and pregnant women with normal glucose tolerance were enrolled in the study, and fecal samples were collected during T2 (weeks 2428) and T3 (weeks 3438). Fecal samples were analyzed from 49 women with GDM and 42 pregnant women with normal glucose tolerance. The 16S rRNA gene amplicon libraries were sequenced to analyze the microbiota and QIIME2 was used to analyze microbiome bioinformatics.
The four dominant phyla that , , and which accomplish about 99% of the total relative abundance did not significantly change between the T2 and T3 in the GDM and healthy groups. At the genus level, the relative abundance of (0 vs. 0.25%, P = 0.041) and (0 vs. 0.29%, P = 0.041) increased significantly in the control group, but not in the GDM group. At the phylum level, the relative abundance of and was significantly different between women with GDM and pregnant women with normal glucose tolerance in both T2 and T3. In T2 and T3, the relative abundances of , , and were significantly higher in the GDM group than in the control group (P<0.05). The relative abundance of in the GDM group was lower than in the control group in both T2 and T3.
The intestinal microbiota composition was stable from T2 to T3 in the GDM and control groups; however, the intestinal microbiota composition was different between the two groups.
本研究旨在探讨妊娠期糖尿病（GDM）妇女和糖耐量正常孕妇在妊娠中期（T2）和妊娠晚期（T3）之间肠道微生物组的组成及其纵向变化。
本观察性研究在北京协和医院进行。纳入 GDM 妇女和糖耐量正常孕妇，在 T2（2428 周）和 T3（3438 周）时收集粪便样本。共分析了 49 例 GDM 妇女和 42 例糖耐量正常孕妇的粪便样本。通过 16S rRNA 基因扩增子文库进行测序分析微生物群，使用 QIIME2 进行微生物组生物信息学分析。
占总相对丰度 99%左右的四个主要菌门 、 、 、 ，在 GDM 和健康组的 T2 和 T3 之间没有显著变化。在属水平上，对照组中 （0 对 0.25%，P = 0.041）和 （0 对 0.29%，P = 0.041）的相对丰度显著增加，但 GDM 组没有。在门水平上，GDM 妇女和糖耐量正常孕妇的 、 相对丰度在 T2 和 T3 之间存在显著差异。在 T2 和 T3 时，GDM 组的 、 、 相对丰度均明显高于对照组（P<0.05）。GDM 组的 相对丰度在 T2 和 T3 时均低于对照组。
GDM 组和对照组从 T2 到 T3 的肠道微生物组组成稳定；然而，两组之间的肠道微生物组组成不同。
