Song Xinjie, Shukla Shruti, Lee Gibaek, Park Sunhyun, Kim Myunghee
Department of Food Science and Technology, Yeungnam University Gyeongsan, South Korea.
Food Standard Research Center, Korea Food Research Institute Seongnam, South Korea.
Front Microbiol. 2016 Jul 21;7:1124. doi: 10.3389/fmicb.2016.01124. eCollection 2016.
Cronobacter species (Cronobacter spp.) are hazardous foodborne pathogens associated with baby food, powdered infant formula (PIF). To develop a rapid and sensitive method for simultaneous detection of seven Cronobacter spp. in PIF, an indirect non-competitive enzyme-linked immunosorbent assay (INC-ELISA) was developed based on a novel immunoglobulin G (IgG), anti-Cronobacter IgG. The developed INC-ELISA was able to detect seven Cronobacter spp. at concentrations ranging from (5.6 ± 0.30) × 10(3) to (2.1 ± 0.01) × 10(5) colony forming unit (CFU)/mL in pure culture. Further, INC-ELISA employing anti-Cronobacter IgG was applicable for analysis of PIF samples contaminated with less than <10 cells of Cronobacter spp. per 25 g of PIF in 36 h. The developed antibody showed slight cross-reactivity with Franconibacter pulveris (LMG 24057) at high concentration (10(8) CFU/mL). The INC-ELISA method displayed excellent specificity without compromising cross-reactivity with other foodborne pathogens. The INC-ELISA assay method developed in this study using a novel anti-Cronobacter IgG facilitated highly sensitive, efficient, and rapid detection of Cronobacter spp. in baby food.
阪崎肠杆菌属（阪崎肠杆菌）是与婴儿食品、婴儿配方奶粉相关的危险食源性病原体。为开发一种快速灵敏的方法用于同时检测婴儿配方奶粉中的7种阪崎肠杆菌，基于一种新型免疫球蛋白G（IgG）、抗阪崎肠杆菌IgG，开发了一种间接非竞争性酶联免疫吸附测定法（INC-ELISA）。所开发的INC-ELISA能够检测纯培养物中浓度范围为（5.6±0.30）×10³至（2.1±0.01）×10⁵菌落形成单位（CFU）/mL的7种阪崎肠杆菌。此外，采用抗阪崎肠杆菌IgG的INC-ELISA适用于分析每25 g婴儿配方奶粉中污染少于10个阪崎肠杆菌细胞的婴儿配方奶粉样品，检测时间为36小时。所开发的抗体在高浓度（10⁸CFU/mL）下与粉状弗朗科尼氏菌（LMG 24057）表现出轻微交叉反应。INC-ELISA方法显示出优异的特异性，且不影响与其他食源性病原体的交叉反应性。本研究中使用新型抗阪崎肠杆菌IgG开发的INC-ELISA测定方法有助于对婴儿食品中的阪崎肠杆菌进行高度灵敏、高效且快速的检测。
Zotero 插件
