Yurong Lin, Biaoxue Rong, Wei Li, Zongjuan Ming, Hongyang Shi, Ping Fang, Wenlong Gao, Shuanying Yang, Zongfang Li
Department of Respiratory Medicine, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China.
Department of Statistics and Epidemiology, Medical College, Lanzhou University, Lanzhou, China.
Oncotarget. 2017 Apr 18;8(16):26000-26012. doi: 10.18632/oncotarget.11006.
Stathmin has been investigated as a tumor biomarker because it appear to be associated with tumorigenesis; however, the effect of stathmin in lung adenocarcinoma (LAC) remains poorly understood. The purpose of this study was to examine the expression of stathmin in lung adenocarcinoma, and to disclose the relationship between them. The expression of stathmin was examined by RT-PCR, IHC and Western blot. Furthermore, small interfering RNA (shRNA)-mediated silencing of stathmin was employed in LAC cells to investigate cell proliferation, invasion and apoptosis. In this study, we showed that overexpression of stathmin was significantly associated with poorly differentiated, lymph node metastasis and advance TNM stages of lung adenocarcinoma. And silencing of stathmin expression inhibited the proliferation, migration and invasion of lung adenocarcinoma PC-9 cells, and retarded the growth of PC-9 cells xenografts in nude mice. Additionally, the anticarcinogenic efficacy of stathmin silencing might be involved in P38 and MMP2 signaling pathways. In conclusion, these results showed that stathmin expression was significantly up-regulated in LAC, which may act as a biomarker for LAC. Furthermore, silence of stathmin inhibiting LAC cell growth indicated that stathmin may be a promising molecular target for LAC therapy.
由于Stathmin似乎与肿瘤发生相关，因此已被作为一种肿瘤生物标志物进行研究；然而，Stathmin在肺腺癌（LAC）中的作用仍知之甚少。本研究的目的是检测Stathmin在肺腺癌中的表达，并揭示它们之间的关系。通过RT-PCR、免疫组化（IHC）和蛋白质免疫印迹法检测Stathmin的表达。此外，在LAC细胞中采用小干扰RNA（shRNA）介导的Stathmin沉默来研究细胞增殖、侵袭和凋亡。在本研究中，我们发现Stathmin的过表达与肺腺癌的低分化、淋巴结转移及进展期TNM分期显著相关。Stathmin表达的沉默抑制了肺腺癌PC-9细胞的增殖、迁移和侵袭，并延缓了PC-9细胞在裸鼠体内异种移植瘤的生长。此外，Stathmin沉默的抗癌作用可能与P38和MMP2信号通路有关。总之，这些结果表明，Stathmin在LAC中的表达显著上调，其可能作为LAC的一种生物标志物。此外，Stathmin沉默抑制LAC细胞生长表明，Stathmin可能是LAC治疗的一个有前景的分子靶点。
