Panduwawala C P, Zhan X, Dissanayaka W L, Samaranayake L P, Jin L, Zhang C
Comprehensive Dental Care (Endodontics), Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
Periodontology, Fujian Medical University School of Stomatology, Fujian, China.
J Periodontal Res. 2017 Jun;52(3):408-418. doi: 10.1111/jre.12405. Epub 2016 Aug 6.
Chronic periodontitis causes damage to tooth-supporting tissues, resulting in tooth loss in adults. Recently, cell-sheet-based approaches have been studied to overcome the limitations of conventional cytotherapeutic procedures for periodontal regeneration. The purpose of the present study was to investigate the regenerative potential of periodontal ligament stem cells (PDLSCs) and human umbilical vein endothelial cells (HUVECs) in three-dimensional (3D) cell sheet constructs for periodontal regeneration in vivo.
PDLSCs, HUVECs or co-cultures of both cells were seeded onto temperature-responsive culture dishes, and intact cell sheets were fabricated. Cell sheets were wrapped around the prepared human roots in three different combinations and implanted subcutaneously into immunodeficient mice.
Histological evaluation revealed that after 2, 4 and 8 wk of implantation, periodontal ligament-like tissue arrangements were observed around the implanted roots in experimental groups compared with controls. Vascular lumens were also observed in periodontal compartments of HUVEC-containing groups. Periodontal ligament regeneration, cementogenesis and osteogenesis were evident in the experimental groups at both weeks 4 and 8, as shown by immunostaining for periostin and bone sialoprotein. Human cells in the transplanted cell sheets were stained by immunohistochemistry for the presence of human mitochondria.
The 3D cell sheet-based approach may be potentially beneficial and is thus encouraged for future regenerative periodontal therapy.
慢性牙周炎会对牙齿支持组织造成损害，导致成年人牙齿缺失。近来，基于细胞片的方法已被用于研究，以克服传统细胞治疗程序在牙周再生方面的局限性。本研究的目的是调查牙周膜干细胞（PDLSCs）和人脐静脉内皮细胞（HUVECs）在三维（3D）细胞片构建物中对体内牙周再生的再生潜力。
将PDLSCs、HUVECs或两种细胞的共培养物接种到温度响应培养皿上，制成完整的细胞片。细胞片以三种不同组合包裹在制备好的人牙根周围，并皮下植入免疫缺陷小鼠体内。
组织学评估显示，植入2、4和8周后，与对照组相比，实验组植入牙根周围观察到牙周膜样组织排列。在含HUVECs的组的牙周区域也观察到血管腔。如骨膜蛋白和骨涎蛋白的免疫染色所示，在第4周和第8周时，实验组的牙周膜再生、牙骨质生成和成骨均很明显。通过免疫组织化学染色检测移植细胞片中的人线粒体，以确定人细胞的存在。
基于三维细胞片的方法可能具有潜在益处，因此鼓励在未来的牙周再生治疗中应用。
