Casarrubios Laura, Matesanz María Concepción, Sánchez-Salcedo Sandra, Arcos Daniel, Vallet-Regí María, Portolés María Teresa
Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense de Madrid, Spain; Instituto de Investigación Sanitaria San Carlos IdISSC, Spain.
Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense de Madrid, Spain.
J Colloid Interface Sci. 2016 Nov 15;482:112-120. doi: 10.1016/j.jcis.2016.07.075. Epub 2016 Jul 29.
Silicon substituted hydroxyapatites (SiHA) are highly crystalline bioceramics treated at high temperatures (about 1200°C) which have been approved for clinical use with spinal, orthopedic, periodontal, oral and craniomaxillofacial applications. The preparation of SiHA with lower temperature methods (about 700°C) provides nanocrystalline SiHA (nano-SiHA) with enhanced bioreactivity due to higher surface area and smaller crystal size. The aim of this study has been to know the nanocrystallinity effects on the response of both osteoblasts and osteoclasts (the two main cell types involved in bone remodelling) to silicon substituted hydroxyapatite.
Saos-2 osteoblasts and osteoclast-like cells (differentiated from RAW-264.7 macrophages) have been cultured on the surface of nano-SiHA and SiHA disks and different cell parameters have been evaluated: cell adhesion, proliferation, viability, intracellular content of reactive oxygen species, cell cycle phases, apoptosis, cell morphology, osteoclast-like cell differentiation and resorptive activity.
This comparative in vitro study evidences that nanocrystallinity of SiHA affects the cell/biomaterial interface inducing bone cell apoptosis by loss of cell anchorage (anoikis), delaying osteoclast-like cell differentiation and decreasing the resorptive activity of this cell type. These results suggest the potential use of nano-SiHA biomaterial for preventing bone resorption in treatment of osteoporotic bone.
硅取代羟基磷灰石（SiHA）是经过高温（约1200°C）处理的高度结晶生物陶瓷，已被批准用于脊柱、骨科、牙周、口腔和颅颌面应用的临床治疗。采用较低温度方法（约700°C）制备SiHA可得到纳米晶SiHA（nano-SiHA），由于其具有更大的表面积和更小的晶体尺寸，因而具有更高的生物活性。本研究的目的是了解纳米晶对成骨细胞和破骨细胞（参与骨重塑的两种主要细胞类型）对硅取代羟基磷灰石反应的影响。
将Saos-2成骨细胞和破骨细胞样细胞（由RAW-264.7巨噬细胞分化而来）培养在nano-SiHA和SiHA圆盘表面，并评估不同的细胞参数：细胞黏附、增殖、活力、细胞内活性氧含量、细胞周期阶段、凋亡、细胞形态、破骨细胞样细胞分化和吸收活性。
这项体外对比研究表明，SiHA的纳米晶性会影响细胞/生物材料界面，通过细胞锚定丧失（失巢凋亡）诱导骨细胞凋亡，延迟破骨细胞样细胞分化，并降低该细胞类型的吸收活性。这些结果表明，nano-SiHA生物材料在治疗骨质疏松性骨病中预防骨吸收方面具有潜在用途。
