De Luca Arnaud, Hankard Régis, Alexandre-Gouabau Marie-Cécile, Ferchaud-Roucher Véronique, Darmaun Dominique, Boquien Clair-Yves
Inserm U 1069, Tours; CHU Tours, Tours, France.
Inserm U 1069, Tours; CHU Tours, Tours, France; Université F Rabelais, Tours, France.
Nutrition. 2016 Nov-Dec;32(11-12):1295-8. doi: 10.1016/j.nut.2016.05.013. Epub 2016 Jun 8.
Nutrition during fetal life and early childhood is thought to play a crucial role in the risk for developing metabolic syndrome and cardiovascular diseases in the future adult and branched-chain amino acids (BCAA) intake may play a role in the development of obesity. The aim of this study was to compare the breast milk amino acid profiles of obese and normal weight (control) breast-feeding mothers.
Fifty obese and 50 control breast-feeding mothers were enrolled. Age and parity were similar in both groups. Breast milk samples were collected at the end of the first month of lactation. Free amino acid (FAA) concentrations in breast milk were determined by ultra-performance liquid chromatography tandem mass spectrometry. Comparisons between groups were performed using a two-tailed paired t test.
We analyzed 45 breast milk samples from each group. Body mass index was 34.3 ± 3.9 kg/m(2) in the obese group and 21.6 ± 1.4 kg/m(2) in the control group (P < 10(-4)). BCAA concentrations were higher in breast milk of obese mothers (95.5 ± 38.2 μM versus 79.8 ± 30.9 μM; P = 0.037), as was tyrosine concentration (13.8 ± 7.1 μM versus 10.6 ± 5.2 μM; P = 0.016).
The mature breast milk of obese mothers contained 20% more BCAA and 30% more tyrosine than breast milk of control mothers. Whether altered breast milk FAA profile affects metabolic risk in the breast-fed child remains to be explored.
胎儿期和幼儿期的营养状况被认为对未来成年人患代谢综合征和心血管疾病的风险起着关键作用，而支链氨基酸（BCAA）的摄入可能在肥胖的发生发展中发挥作用。本研究旨在比较肥胖和正常体重（对照）哺乳期母亲的母乳氨基酸谱。
招募了50名肥胖哺乳期母亲和50名对照哺乳期母亲。两组的年龄和胎次相似。在哺乳第一个月末采集母乳样本。采用超高效液相色谱串联质谱法测定母乳中的游离氨基酸（FAA）浓度。组间比较采用双侧配对t检验。
我们分析了每组45份母乳样本。肥胖组的体重指数为34.3±3.9kg/m²，对照组为21.6±1.4kg/m²（P<10⁻⁴）。肥胖母亲母乳中的BCAA浓度较高（95.5±38.2μM对79.8±30.9μM；P=0.037），酪氨酸浓度也较高（13.8±7.1μM对10.6±5.2μM；P=0.016）。
肥胖母亲的成熟母乳中BCAA含量比对照母亲的母乳多20%，酪氨酸含量多30%。母乳FAA谱的改变是否会影响母乳喂养儿童的代谢风险仍有待探索。
