Lewis D C, Shibamoto T
Department of Environmental Toxicology, University of California, Davis 95616.
Toxicon. 1989;27(5):519-29. doi: 10.1016/0041-0101(89)90113-x.
Naturally occurring quinones and quinone-containing extracts of seeds of the toxic plant Cassia obtusifolia (sicklepod) affected muscle mitochondrial function. Aqueous suspensions and organic extracts of C. obtusifolia seeds slightly elevated plasma creatine kinase levels of Sprague-Dawley rats. These extracts were analyzed by fused silica capillary gas chromatography and found to contain nine anthraquinones and three anthrones. Urinary metabolites primarily consisted of beta-glucuronide conjugates of the anthraquinones. The three anthrones or conjugate analogues were not present in the urine in detectable amounts. Emodin, doxorubicin and organic extracts of C. obtusifolia inhibited NADH:cytochrome c oxidoreductase activity of bovine heart mitochondrial particles and NADH:CoQ oxidoreductase activity of porcine heart mitochondrial NADH dehydrogenase, whereas juglone was stimulatory. Relative quinone metabolism correlated with semiquinone formation rate and with redox potential. A protective effect of coenzyme Q against enzyme inhibition by anthraquinones was also observed.
天然存在的醌类以及有毒植物决明（望江南）种子中含醌的提取物会影响肌肉线粒体功能。决明种子的水悬浮液和有机提取物会使斯普拉格-道利大鼠的血浆肌酸激酶水平略有升高。这些提取物通过熔融石英毛细管气相色谱法进行分析，发现含有9种蒽醌和3种蒽酮。尿液代谢物主要由蒽醌的β-葡萄糖醛酸苷缀合物组成。三种蒽酮或缀合物类似物在尿液中未检测到。大黄素、阿霉素和决明的有机提取物抑制牛心线粒体颗粒的NADH：细胞色素c氧化还原酶活性以及猪心线粒体NADH脱氢酶的NADH：辅酶Q氧化还原酶活性，而胡桃醌具有刺激作用。相对醌代谢与半醌形成速率和氧化还原电位相关。还观察到辅酶Q对抗蒽醌对酶的抑制作用。
