Golmohammadi Rahim, Sabaghzadeh Fatemeh, Mojadadi Mohammad Shafi
Department of of Anatomy, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, I.R. Iran.
Department of Biochemistry, Faculty of Basic Sciences, Hakim Sabzevari University, Sabzevar, I.R. Iran.
Res Pharm Sci. 2016 May-Jun;11(3):227-32.
Anethum graveolens or Dill (local name: Shevid) belongs to the family of Apiaceae (Umbelliferae) and is used traditionally for the treatment of convulsion and diabetes in Iran. This study aimed to investigate the effect of hydroalcoholic extract of A. graveolens leaves on the histology of the dentate gyrus of the hippocampus in the epileptic mice kindled by Pentylenetetrazole (PTZ). In this experimental study, the epileptic BALB/c mice kindled by PTZ were randomly divided into four groups of 10 animals each. Three experimental groups received 250, 500 and 750 mg/kg/day of A. graveolens extract for 21 days. The control group received phosphate-buffered saline (PBS). After the treatment period, the mice were anesthetized, and their hippocampi were dissected for the histopathological analysis, and immunohistochemical analysis for caspase-3 activity. Histopathological examinations showed that the mean numbers of the healthy neuronal cells in the dentate gyrus of the mice received 500 mg/kg/day of A. graveolens extracts were significantly higher than those of the mice received 250 and 750 mg/kg/day of the extracts as well as the control group (P < 0.05 and P < 0.001, respectively). In addition, the results of immunohistochemical analysis revealed that in mice treated with 500 mg/kg/day of A. graveolens; the numbers of caspase-3-positive cells in the dentate gyrus were significantly lower than those of the two other test and the control groups. The findings of this study suggest that 500 mg/kg/day of the A. graveolens extract could have protective effect on the dentate gyrus of the hippocampus in the epileptic mice.
莳萝（学名：Anethum graveolens，当地名称：Shevid）属于伞形科，在伊朗传统上用于治疗惊厥和糖尿病。本研究旨在探讨莳萝叶水醇提取物对戊四氮（PTZ）点燃的癫痫小鼠海马齿状回组织学的影响。在本实验研究中，将PTZ点燃的癫痫BALB/c小鼠随机分为四组，每组10只动物。三个实验组分别接受250、500和750毫克/千克/天的莳萝提取物，持续21天。对照组接受磷酸盐缓冲盐水（PBS）。治疗期结束后，将小鼠麻醉，解剖其海马进行组织病理学分析和caspase-3活性的免疫组织化学分析。组织病理学检查显示，接受500毫克/千克/天莳萝提取物的小鼠齿状回中健康神经元细胞的平均数量显著高于接受250和750毫克/千克/天提取物的小鼠以及对照组（分别为P < 0.05和P < 0.001）。此外，免疫组织化学分析结果显示，在接受500毫克/千克/天莳萝治疗的小鼠中，齿状回中caspase-3阳性细胞的数量显著低于其他两个测试组和对照组。本研究结果表明，500毫克/千克/天的莳萝提取物可能对癫痫小鼠海马齿状回具有保护作用。
