Poderys Vilius, Matulionyte Marija, Selskis Algirdas, Rotomskis Ricardas
Laboratory of Biomedical Physics, Vilnius University Institute of Oncology, Vilnius, Lithuania.
Biophotonics Laboratory, Quantum Electronics Department, Physics Faculty, Vilnius University, Vilnius, Lithuania.
Nanoscale Res Lett. 2011 Dec;6(1):9. doi: 10.1007/s11671-010-9740-9. Epub 2010 Aug 22.
Semiconductor nanoparticles (quantum dots) are promising fluorescent markers, but it is very little known about interaction of quantum dots with biological molecules. In this study, interaction of CdTe quantum dots coated with thioglycolic acid (TGA) with bovine serum albumin was investigated. Steady state spectroscopy, atomic force microscopy, electron microscopy and dynamic light scattering methods were used. It was explored how bovine serum albumin affects stability and spectral properties of quantum dots in aqueous media. CdTe-TGA quantum dots in aqueous solution appeared to be not stable and precipitated. Interaction with bovine serum albumin significantly enhanced stability and photoluminescence quantum yield of quantum dots and prevented quantum dots from aggregating.
半导体纳米颗粒（量子点）是很有前景的荧光标记物，但关于量子点与生物分子的相互作用却知之甚少。在本研究中，研究了用巯基乙酸（TGA）包覆的CdTe量子点与牛血清白蛋白的相互作用。使用了稳态光谱、原子力显微镜、电子显微镜和动态光散射方法。探究了牛血清白蛋白如何影响量子点在水性介质中的稳定性和光谱性质。水溶液中的CdTe-TGA量子点似乎不稳定且会沉淀。与牛血清白蛋白的相互作用显著提高了量子点的稳定性和光致发光量子产率，并防止了量子点聚集。
