Yong Hoi-Sen, Song Sze-Looi, Lim Phaik-Eem, Eamsobhana Praphathip, Suana I Wayan
Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia.
Chancellory High Impact Research, University of Malaya, Kuala Lumpur, Malaysia.
Genetica. 2016 Oct;144(5):513-521. doi: 10.1007/s10709-016-9919-9. Epub 2016 Aug 9.
Zeugodacus caudatus is a pest of pumpkin flowers. It has a Palearctic and Oriental distribution. We report here the complete mitochondrial genome of the Malaysian and Indonesian samples of Z. caudatus determined by next-generation sequencing of genomic DNA and determine their taxonomic status as sibling species and phylogeny with other taxa of the genus Zeugodacus. The whole mitogenome of both samples possessed 37 genes (13 protein-coding genes-PCGs, 2 rRNA and 22 tRNA genes) and a control region. The mitogenome of the Indonesian sample (15,885 bp) was longer than that of the Malaysian sample (15,866 bp). In both samples, TΨC-loop was absent in trnF and DHU-loop was absent in trnS1. Molecular phylogeny based on 13 PCGs was concordant with 15 mitochondrial genes (13 PCGs and 2 rRNA genes), with the two samples of Z. caudatus forming a sister group and the genus Zeugodacus was monophyletic. The Malaysian and Indonesian samples of Z. caudatus have a genetic distance of p = 7.8 % based on 13 PCGs and p = 7.0 % based on 15 mitochondrial genes, indicating status of sibling species. They are proposed to be accorded specific status as members of a species complex.
瓜实蝇是南瓜花的一种害虫。它分布于古北区和东洋区。我们在此报告通过对基因组DNA进行二代测序确定的马来西亚和印度尼西亚瓜实蝇样本的完整线粒体基因组，并确定它们作为姐妹种的分类地位以及与瓜实蝇属其他类群的系统发育关系。两个样本的完整线粒体基因组均包含37个基因（13个蛋白质编码基因、2个rRNA基因和22个tRNA基因）以及一个控制区。印度尼西亚样本的线粒体基因组（15,885 bp）比马来西亚样本的（15,866 bp）长。在两个样本中，trnF中缺少TΨC环，trnS1中缺少DHU环。基于13个蛋白质编码基因的分子系统发育与基于15个线粒体基因（13个蛋白质编码基因和2个rRNA基因）的结果一致，瓜实蝇的两个样本形成一个姐妹群，瓜实蝇属是单系的。基于13个蛋白质编码基因，马来西亚和印度尼西亚瓜实蝇样本的遗传距离为p = 7.8%，基于15个线粒体基因的遗传距离为p = 7.0%，表明它们为姐妹种。它们被提议作为一个物种复合体的成员给予特定地位。
