Yong Hoi-Sen, Song Sze-Looi, Lim Phaik-Eem, Eamsobhana Praphathip
Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Institute of Ocean and Earth Sciences, University of Malaya, Kuala Lumpur, Malaysia.
PLoS One. 2017 Dec 7;12(12):e0189325. doi: 10.1371/journal.pone.0189325. eCollection 2017.
The tephritid fruit fly Zeugodacus tau (Walker) is a polyphagous fruit pest of economic importance in Asia. Studies based on genetic markers indicate that it forms a species complex. We report here (1) the complete mitogenome of Z. tau from Malaysia and comparison with that of China as well as the mitogenome of other congeners, and (2) the relationship of Z. tau taxa from different geographical regions based on sequences of cytochrome c oxidase subunit I gene. The complete mitogenome of Z. tau had a total length of 15631 bp for the Malaysian specimen (ZT3) and 15835 bp for the China specimen (ZT1), with similar gene order comprising 37 genes (13 protein-coding genes-PCGs, 2 rRNA genes, and 22 tRNA genes) and a non-coding A + T-rich control region (D-loop). Based on 13 PCGs and 15 mt-genes, Z. tau NC_027290 (China) and Z. tau ZT1 (China) formed a sister group in the lineage containing also Z. tau ZT3 (Malaysia). Phylogenetic analysis based on partial sequences of cox1 gene indicates that the taxa from China, Japan, Laos, Malaysia, Bangladesh, India, Sri Lanka, and Z. tau sp. A from Thailand belong to Z. tau sensu stricto. A complete cox1 gene (or 13 PCGs or 15 mt-genes) instead of partial sequence is more appropriate for determining phylogenetic relationship.
实蝇科果实蝇Zeugodacus tau（沃克）是亚洲一种具有经济重要性的多食性果实害虫。基于遗传标记的研究表明它形成了一个物种复合体。我们在此报告：（1）来自马来西亚的Z. tau的完整线粒体基因组，并与来自中国的以及其他同属物种的线粒体基因组进行比较，以及（2）基于细胞色素c氧化酶亚基I基因序列分析不同地理区域的Z. tau分类群之间的关系。马来西亚标本（ZT3）的Z. tau完整线粒体基因组全长为15631 bp，中国标本（ZT1）的为15835 bp，基因排列相似，包含37个基因（13个蛋白质编码基因、2个rRNA基因和22个tRNA基因）以及一个非编码的富含A + T的控制区（D环）。基于13个蛋白质编码基因和15个线粒体基因，Z. tau NC_027290（中国）和Z. tau ZT1（中国）在一个谱系中形成了一个姐妹群，该谱系中还包括Z. tau ZT3（马来西亚）。基于cox1基因部分序列的系统发育分析表明，来自中国、日本、老挝、马来西亚、孟加拉国、印度、斯里兰卡的分类群以及来自泰国的Z. tau sp. A属于狭义的Z. tau。使用完整而非部分序列的cox1基因（或13个蛋白质编码基因或15个线粒体基因）来确定系统发育关系更为合适。
