Vera-Sánchez Mar, Aznar-Cervantes Salvador, Jover Eva, García-Bernal David, Oñate-Sánchez Ricardo E, Hernández-Romero Diana, Moraleda Jose M, Collado-González Mar, Rodríguez-Lozano Francisco Javier, Cenis Jose Luis
1 Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB, University of Murcia , Murcia, Spain .
2 Biotechnology Department, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA) , Murcia, Spain .
Stem Cells Dev. 2016 Nov 15;25(22):1742-1754. doi: 10.1089/scd.2016.0028. Epub 2016 Aug 8.
Graphene represents one of the most interesting additions to the tissue engineering toolbox. Novel graphene-based composites are required to improve the beneficial graphene properties in terms of tridimensional polymeric structure, conferring a higher mechanical strength and favoring the differentiation of human mesenchymal stem cells. Here, we have demonstrated in a wide range of composite combinations, the successful use of graphene and silk-fibroin constructs for future bioengineering applications in the field of clinical regenerative dentistry using human periodontal ligament stem cells. Our results provide exciting new data for the development of suitable scaffolds that allow good cell engrafting, preservation of cell viability and proliferation, promotion of spontaneous osteoblastic differentiation, and importantly, stimulation of a higher cementum physiological synthesis than using other different available biomaterials.
石墨烯是组织工程工具库中最有趣的新增材料之一。需要新型的基于石墨烯的复合材料，以在三维聚合物结构方面改善石墨烯的有益特性，赋予更高的机械强度，并有利于人间充质干细胞的分化。在这里，我们已经在广泛的复合材料组合中证明，使用人牙周膜干细胞，石墨烯和丝素蛋白构建体可成功用于临床再生牙科领域未来的生物工程应用。我们的结果为开发合适的支架提供了令人兴奋的新数据，这些支架能够实现良好的细胞植入、保持细胞活力和增殖、促进自发成骨细胞分化，重要的是，与使用其他不同的现有生物材料相比，能刺激更高的牙骨质生理合成。
