Aksu Sertan, Çalışkan Eray, Cakiroglu Yigit
American Hospital, Istanbul, Turkey.
Bahcesehir University, Istanbul, Turkey.
J Reprod Immunol. 2016 Sep;117:70-5. doi: 10.1016/j.jri.2016.07.004. Epub 2016 Aug 1.
To determine whether natural killer cell (NK) concentrations in the mid-secretory endometrial tissue of women with unexplained infertility differ from those in the mid-secretory endometrial tissue of fertile women.
This study was conducted with 22 patients with unexplained infertility caused by unsuccessful ovulation induced with gonadotrophins and 12 healthy fertile women, who formed a control group. Mid-secretory endometrial tissue samples were obtained with a Pipelle catheter, and the endometrial NK cell phenotypes were determined by flow cytometry.
The median number of CD4+ cells in the endometrium was significantly lower in women with unexplained infertility compared with in the fertile controls (3.31 vs. 5.81; p=0.02). The CD4(+)/CD8(+) cell ratio in the unexplained-infertility group was significantly lower than that in the control group (0.42 vs. 0.76, p=0.01). The median number of CD103(+) cells in the endometrium of the infertile group was significantly lower than that in the endometrium of the control group (4.40 vs. 6.73, p=0.02). The median number of CD16(+) cells was significantly higher in infertile women than in control women (0.12 vs. 0.001, p=0.01).
Significant reductions in the numbers of CD4(+) and CD103(+) cells as well as in the CD4(+)/CD8(+) ratio but an increased number of CD16(+) cells in the endometrium of infertile women suggest that disordered NK cell activity may be a contributor to the etiology of unexplained infertility.
确定不明原因不孕症女性分泌中期子宫内膜组织中的自然杀伤细胞（NK）浓度是否与有生育能力女性分泌中期子宫内膜组织中的NK浓度不同。
本研究纳入了22例因促性腺激素诱导排卵未成功而导致不明原因不孕症的患者以及12名健康有生育能力的女性作为对照组。使用Pipelle导管获取分泌中期子宫内膜组织样本，并通过流式细胞术测定子宫内膜NK细胞表型。
与有生育能力的对照组相比，不明原因不孕症女性子宫内膜中CD4+细胞的中位数显著降低（3.31对5.81；p = 0.02）。不明原因不孕症组的CD4(+)/CD8(+)细胞比率显著低于对照组（0.42对0.76，p = 0.01）。不孕组子宫内膜中CD103(+)细胞的中位数显著低于对照组子宫内膜中的中位数（4.40对6.73，p = 0.02）。不孕女性中CD16(+)细胞的中位数显著高于对照女性（0.12对0.001，p = 0.01）。
不孕女性子宫内膜中CD4(+)和CD103(+)细胞数量、CD4(+)/CD8(+)比率显著降低，但CD16(+)细胞数量增加，这表明NK细胞活性紊乱可能是不明原因不孕症病因的一个因素。
