Brarda O A, Vanella L M, Greco C R
Department of Microbiology and Immunology, National University of Rio Cuarto, Argentina.
Allergol Immunopathol (Madr). 1989 Jan-Feb;17(1):45-8.
The relationship between ingestion of cow's milk and lymphocyte transformation in cow's milk allergic children was studied. Fifty-six children were selected. Nineteen of them suffered from cow's milk hypersensitivity (HCM); 22 were allergic to several agents except to cow's milk (HOE) and 15 normal and healthy children composed the control group (NH). Lymphoblastogenesis was measured by tritiaded thymidine uptake and the cells were cultured with plasma from the same individual. The lymphoblastogenesis was indirectly estimated by the following equation: (formula; see text) where cpm I represents lymphoblastogenesis estimulated by PHA-M and cpm E represents lymphoblastic transformation with no antigen added to the culture medium. BTI was determined before and 30 days after the elimination of cow's milk from the diet. Lymphoblastogenesis, in the samples obtained before the elimination diet, was significantly increased (BTI decreased) in HCM group but not in the others (p less than 0.01). There was no difference in the lymphoblastogenesis behaviour of the three groups after 30 days of exclusion diet. The difference between the BTI values obtained before and after the elimination cow's milk from the diet would be helpful in the early diagnosis of cow's milk allergy.
研究了牛奶过敏儿童摄入牛奶与淋巴细胞转化之间的关系。选取了56名儿童。其中19名患有牛奶超敏反应（HCM）；22名对除牛奶外的几种物质过敏（HOE），15名正常健康儿童组成对照组（NH）。通过氚标记胸腺嘧啶核苷摄取来测量淋巴细胞生成，细胞用来自同一个体的血浆培养。淋巴细胞生成通过以下公式间接估算：（公式；见正文）其中cpm I表示由PHA - M刺激的淋巴细胞生成，cpm E表示在培养基中未添加抗原时的淋巴细胞转化。在从饮食中去除牛奶之前和之后30天测定BTI。在去除饮食前获得的样本中，HCM组的淋巴细胞生成显著增加（BTI降低），而其他组则没有（p小于0.01）。排除饮食30天后，三组的淋巴细胞生成行为没有差异。从饮食中去除牛奶前后获得的BTI值之间的差异有助于牛奶过敏的早期诊断。
