Vanella L, de González Lascano A M
Department of Microbiology and Immunology, National University of Rio Cuarto, Argentina.
Allergol Immunopathol (Madr). 1988 Sep-Oct;16(5):327-31.
Three groups of infants were formed according to the type of feeding: I-breast milk from mothers without any kind of elimination diet, II-cow's milk-based formula, and III-breast milk till 2 months of age and after that, cow's milk formula. CD4 and CD8 were determined by monoclonal antibodies technique, IgE by ELISA and prick test with either intact or enzymatically digested cow's milk antigens. The average age of onset of the atopic disease in the first group was 2m 10d +/- 15d, in the second: 3m 4d +/- 14d, and in the third: 2m 11d +/- 1m 18d. The number of CD4 in group II was the lowest while in group III was the highest. CD8 value in the second group was higher than in the others. Ratio between CD4 and CD8 in cow's milk-fed infants was the lowest. Total serum IgE level in group II was the lowest although the mean values of the three groups were significantly increased. Prick tests, with at least one antigen, were positive in 75%, 50% and 48% in group I, II and III respectively. It is suggested that the behaviour of the parameters studied would be related to the dose of cow's milk proteins received through breast milk in breast-fed infants; to the relatively great amount of cow's milk antigens and to the lack of breast-feeding in infants fed on cow's milk formula; and to the interaction of such factors in infants fed with both cow's milk and breast milk.
I组——来自未进行任何排除饮食的母亲的母乳；II组——以牛奶为基础的配方奶；III组——2个月龄前喂母乳，之后喂牛奶配方奶。采用单克隆抗体技术测定CD4和CD8，采用酶联免疫吸附测定法（ELISA）和用完整或酶消化的牛奶抗原进行点刺试验测定IgE。第一组特应性疾病的平均发病年龄为2个月10天±15天，第二组为3个月4天±14天，第三组为2个月11天±1个月18天。II组的CD4数量最低，而III组最高。第二组的CD8值高于其他组。牛奶喂养婴儿的CD4与CD8之比最低。II组的总血清IgE水平最低，尽管三组的平均值均显著升高。I组、II组和III组中，至少对一种抗原进行点刺试验呈阳性的比例分别为75%、50%和48%。研究表明，所研究参数的变化可能与母乳喂养婴儿通过母乳摄入的牛奶蛋白剂量有关；与牛奶抗原相对大量以及牛奶配方奶喂养婴儿缺乏母乳喂养有关；还与同时接受牛奶和母乳喂养的婴儿中这些因素的相互作用有关。
