Kolawole T A, Oyeyemi W A, Adigwe C, Leko B, Udeh C, Dapper D V
Department of Physiology, Madonna University, Elele Campus, Rivers State.
Niger J Physiol Sci. 2015 Dec 20;30(1-2):11-6.
Effect of honey on reproductive functions of male rats exposed to nicotine was examined in this study. Thirty-two adult male wistar rats (n=8/Group) were grouped as Control (distilled water), Nicotine (1.0mg/kg bwt), Honey (100mg/kg bwt) and Nicotine with Honey. The animals were orally treated for 35 days consecutively. Epididymis sperm motility, viability, morphology and counts were estimated, serum Follicle Stimulating Hormone (FSH), Leutinizing Hormone (LH) and Testosterone were assayed using ELISA method and testicular histology were also assessed. Significant reduction in percentage sperm motility, viability, morphology and counts were observed in nicotine group compared to control. Serum FSH, LH and testosterone levels were significantly reduced in nicotine group when compared with the control. There was significant improvement in sperm motility, viability, morphology, counts, FSH, LH and Testosterone in group co-treated with nicotine and honey relative to nicotine group. Also, the degenerative seminiferous tubule architecture due to nicotine was improved by honey. In conclusion, honey may suppress nicotine toxic effect on reproductive functions in male Wistar rats.
本研究考察了蜂蜜对暴露于尼古丁的雄性大鼠生殖功能的影响。32只成年雄性Wistar大鼠（每组n = 8只）被分为对照组（蒸馏水）、尼古丁组（1.0mg/kg体重）、蜂蜜组（100mg/kg体重）和尼古丁加蜂蜜组。动物连续35天接受口服处理。评估附睾精子活力、存活率、形态和数量，采用酶联免疫吸附测定（ELISA）法检测血清促卵泡激素（FSH）、促黄体生成素（LH）和睾酮，并评估睾丸组织学。与对照组相比，尼古丁组精子活力、存活率、形态和数量的百分比显著降低。与对照组相比，尼古丁组血清FSH、LH和睾酮水平显著降低。相对于尼古丁组，尼古丁和蜂蜜联合处理组的精子活力、存活率、形态、数量、FSH、LH和睾酮有显著改善。此外，蜂蜜改善了尼古丁导致的生精小管结构退化。总之，蜂蜜可能抑制尼古丁对雄性Wistar大鼠生殖功能的毒性作用。
