Zhang Xiao-Gang, Wang Xi, Zhou Ting-Ting, Wu Xue-Fei, Peng Yan, Zhang Wan-Qin, Li Shao, Zhao Jie
Department of Physiology, Dalian Medical University Dalian, China.
Department of Neurology, the First Affiliated Hospital of Dalian Medical University Dalian, China.
Front Pharmacol. 2016 Jul 26;7:227. doi: 10.3389/fphar.2016.00227. eCollection 2016.
Scorpion venom heat-resistant peptide (SVHRP) is a component purified from Buthus martensii Karsch scorpion venom. Our previous studies found SVHRP could enhance neurogenesis and inhibit microglia-mediated neuroinflammation in vivo. Here, we use the transgenic CL4176, CL2006, and CL2355 strains of Caenorhabditis elegans which express the human Aβ1-42 to investigate the effects and the possible mechanisms of SVHRP mediated protection against Aβ toxicity in vivo. The results showed that SVHRP-fed worms displayed remarkably decreased paralysis, less abundant toxic Aβ oligomers, reduced Aβ plaque deposition with respect to untreated animals. SVHRP also suppressed neuronal Aβ expression-induced defects in chemotaxis behavior and attenuated levels of ROS in the transgenic C. elegans. Taken together, these results suggest SVHRP could protect against Aβ-induced toxicity in C. elegans. Further studies need to be conducted in murine models and humans to analyze the effectiveness of the peptide.
蝎毒耐热肽（SVHRP）是从东亚钳蝎毒液中纯化得到的一种成分。我们之前的研究发现，SVHRP在体内可增强神经发生并抑制小胶质细胞介导的神经炎症。在此，我们使用表达人Aβ1-42的秀丽隐杆线虫转基因CL4176、CL2006和CL2355品系，来研究SVHRP在体内介导的对Aβ毒性的保护作用及其可能机制。结果显示，与未处理的动物相比，喂食SVHRP的线虫麻痹明显减轻，有毒Aβ寡聚体数量减少，Aβ斑块沉积减少。SVHRP还抑制了神经元Aβ表达诱导的趋化行为缺陷，并降低了转基因秀丽隐杆线虫中的活性氧水平。综上所述，这些结果表明SVHRP可保护秀丽隐杆线虫免受Aβ诱导的毒性。需要在小鼠模型和人类中进行进一步研究，以分析该肽的有效性。
