Heidari Pedram, Szretter Alicia, Rushford Laura E, Stevens Maria, Collier Lee, Sore Judit, Hooker Jacob, Mahmood Umar
Department of Radiology, Massachusetts General Hospital Boston, MA 02114, USA.
Am J Nucl Med Mol Imaging. 2016 Jul 6;6(3):176-84. eCollection 2016.
The interest in (68)Gallium labeled PET probes continues to increase around the world. Widespread use in Europe and Asia has led to great interest for use at numerous sites in the US. One barrier to entry is the cost of the automated synthesis units for relatively simple labeling procedures. We describe the construction and testing of a relatively low-cost automated (68)Ga-labeling unit for human-use. We provide a guide for construction, including part lists and synthesis timelists to facilitate local implementation. Such inexpensive systems could help increase use around the globe and in the US in particular by removing one of the barriers to greater widespread availability. The developed automated synthesis unit reproducibly synthesized (68)Ga-DOTATOC with average yield of 71 ± 8% and a radiochemical purity ≥ 95% in a synthesis time of 25 ± 1 minutes. Automated product yields are comparable to that of manual synthesis. We demonstrate in-house construction and use of a low-cost automated synthesis unit for labeling of DOTATOC and similar peptides with (68)Gallium.
全球范围内，对（68）镓标记的正电子发射断层扫描（PET）探针的兴趣持续增长。在欧洲和亚洲的广泛应用引发了美国众多机构对其使用的浓厚兴趣。进入该领域的一个障碍是相对简单标记程序的自动化合成装置成本高昂。我们描述了一种用于人类使用的相对低成本的自动化（68）镓标记装置的构建与测试。我们提供了一份构建指南，包括部件清单和合成时间表，以方便在当地实施。这种价格低廉的系统通过消除更广泛应用的障碍之一，有助于在全球尤其是美国增加其使用。所开发的自动化合成装置能够可重复地合成（68）镓 - 多柔比星，在25 ± 1分钟的合成时间内平均产率为71 ± 8%，放射化学纯度≥ 95%。自动化产品产率与手动合成相当。我们展示了一种低成本自动化合成装置的内部构建及其用于用（68）镓标记多柔比星和类似肽的情况。
