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Reducing the effects of PCR amplification and sequencing artifacts on 16S rRNA-based studies.减少 PCR 扩增和测序伪影对基于 16S rRNA 的研究的影响。
PLoS One. 2011;6(12):e27310. doi: 10.1371/journal.pone.0027310. Epub 2011 Dec 14.
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Appl Environ Microbiol. 2009 Dec;75(23):7537-41. doi: 10.1128/AEM.01541-09. Epub 2009 Oct 2.

沙特阿拉伯麦加地区真菌类群的宏基因组分析。

Metagenomic analysis of fungal taxa inhabiting Mecca region, Saudi Arabia.

作者信息

Moussa Tarek A A, Al-Zahrani Hassan S, Almaghrabi Omar A, Sabry Nevien M, Fuller Michael P

机构信息

Biological Sciences Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia; Biological Sciences Department, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia; Botany and Microbiology Department, Faculty of Science, Cairo University, Giza 12613, Egypt.

Biological Sciences Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.

出版信息

Genom Data. 2016 Jul 21;9:126-7. doi: 10.1016/j.gdata.2016.07.008. eCollection 2016 Sep.

DOI:10.1016/j.gdata.2016.07.008
PMID:27508121
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4971153/
Abstract

The data presented contains the sequences of fungal Internal Transcribed Spacer (ITS) and 18S rRNA gene from a metagenome of the Mecca region, Saudi Arabia. Sequences were amplified using fungal specific primers, which amplified the amplicon aligned between the 18S and 28S rRNA genes. A total of 460 fungal species belonging to 133 genera, 58 families, 33 orders, 13 classes and 4 phyla were identified in four contrasting locations. The raw sequencing data used to perform this analysis along with FASTQ file are located in the NCBI Sequence Read Archive (SRA) under accession numbers: SRR3150823, SRR3144873, SRR3150825 and SRR3150846.

摘要

所呈现的数据包含来自沙特阿拉伯麦加地区宏基因组的真菌内转录间隔区(ITS)和18S rRNA基因序列。使用真菌特异性引物扩增序列,该引物扩增出18S和28S rRNA基因之间比对的扩增子。在四个不同地点共鉴定出460种真菌,分属于133个属、58个科、33个目、13个纲和4个门。用于进行此分析的原始测序数据以及FASTQ文件存于NCBI序列读取存档库(SRA)中,登录号为:SRR3150823、SRR3144873、SRR3150825和SRR3150846。