用于常规饲养和传统饲养斑马鱼幼体的16S rRNA扩增子测序数据集。
16S rRNA amplicon sequencing dataset for conventionalized and conventionally raised zebrafish larvae.
作者信息
Davis Daniel J, Bryda Elizabeth C, Gillespie Catherine H, Ericsson Aaron C
机构信息
Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65201, USA.
Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65201, USA; University of Missouri Metagenomics Center (MUMC), University of Missouri, Columbia, MO 65201, USA.
出版信息
Data Brief. 2016 Jul 5;8:938-43. doi: 10.1016/j.dib.2016.06.057. eCollection 2016 Sep.
Data presented here contains metagenomic analysis regarding the sequential conventionalization of germ-free zebrafish embryos. Zebrafish embryos that underwent a germ-free sterilization process immediately after fertilization were promptly exposed to and raised to larval stage in conventional fish water. At 6 days postfertilization (dpf), these "conventionalized" larvae were compared to zebrafish larvae that were raised in conventional fish water never undergoing the initial sterilization process. Bacterial 16S rRNA amplicon sequencing was performed on DNA isolated from homogenates of the larvae revealing distinct microbiota variations between the two groups. The dataset described here is also related to the research article entitled "Microbial modulation of behavior and stress responses in zebrafish larvae" (Davis et al., 2016) [1].
此处呈现的数据包含关于无菌斑马鱼胚胎序贯定殖的宏基因组分析。受精后立即经过无菌消毒处理的斑马鱼胚胎，随即被置于常规养鱼水中并饲养至幼体阶段。在受精后6天（dpf），将这些“定殖化”幼体与从未经历初始消毒过程、一直在常规养鱼水中饲养的斑马鱼幼体进行比较。对从幼体匀浆中分离出的DNA进行细菌16S rRNA扩增子测序，结果显示两组之间存在明显的微生物群差异。此处描述的数据集也与题为《斑马鱼幼体行为和应激反应的微生物调节》（戴维斯等人，2016年）[1]的研究文章相关。
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