Liang Ying, Liu Haizi, Zhai Shaowei, Huang Lixing, Huang Wenshu, Huang Bei, Xu Jisong, Xiong Jing, Wang Bei
Fisheries College, Jimei University, Xiamen, Fujian, 361021, China.
Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang, Guangdong, 524088, China.
Heliyon. 2023 Jun 2;9(6):e16965. doi: 10.1016/j.heliyon.2023.e16965. eCollection 2023 Jun.
This study aimed to investigate the effects of weaning American glass eels () with the formula diet on intestinal microbiota and the expression of inflammatory cytokines genes. During the feeding trial, the control group (termed IF group) was fed with initial feed for 34 days, and the experimental group (termed FF group) was fed with initial feed for 30 days, and then weaned with the formula diet for 4 days. After feeding trial, intestines were subjected to microbiota analysis using 16S rDNA high-throughput sequencing, and expression of three inflammatory cytokines genes in gut were examined by qPCR. The results indicated that the species richness and diversity of intestinal microbiota exhibited significantly higher in FF group than that in IF group < 0.05). At the phylum level, the core intestinal microflora was the same for two groups. The most abundant phylum was Firmicutes in IF group, while it was Proteobacteria in FF group. Five genera were significantly higher in the IF group compared with the FF group, and was the most major enriched biomarker at genus level. Nine genera were significantly higher in the FF group compared with the IF group, and was the most major enriched biomarker. Weaning from initial feeding diet to formula feeding diet enhanced the expression levels of and , and there was no significant change in expression between the two groups. These findings would be very useful to improve the diet formulation for weaning stage of American glass eels.
本研究旨在探讨用配方饲料对美洲鳗鲡玻璃鳗进行断奶对其肠道微生物群及炎性细胞因子基因表达的影响。在饲养试验期间，对照组（称为IF组）投喂初始饲料34天，实验组（称为FF组）投喂初始饲料30天，然后用配方饲料断奶4天。饲养试验结束后，采用16S rDNA高通量测序对肠道进行微生物群分析，并用qPCR检测肠道中三种炎性细胞因子基因的表达。结果表明，FF组肠道微生物群的物种丰富度和多样性显著高于IF组（P<0.05）。在门水平上，两组的核心肠道微生物区系相同。IF组中最丰富的门是厚壁菌门，而FF组中是变形菌门。与FF组相比，IF组有5个属显著更高，且在属水平上是最主要的富集生物标志物。与IF组相比，FF组有9个属显著更高，且是最主要的富集生物标志物。从初始饲料断奶到配方饲料喂养可提高白细胞介素-1β和肿瘤坏死因子-α的表达水平，两组间白细胞介素-6的表达无显著变化。这些发现对于改进美洲鳗鲡玻璃鳗断奶阶段的饲料配方非常有用。
