阿地骨化醇对多囊卵巢综合征模型细胞色素 P450 相关基因表达、急性类固醇调节蛋白和卵巢结构的影响。
The effects of AdipoRon on cytochrome P450-related gene expression, acute steroidogenic regulatory protein, and structure of ovary in polycystic ovary syndrome model.
机构信息
Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Endometrium and Endometriosis Research Centre, Hamadan University of Medical Sciences, Hamadan, Iran.
出版信息
J Assist Reprod Genet. 2023 Oct;40(10):2453-2461. doi: 10.1007/s10815-023-02900-1. Epub 2023 Sep 5.
PURPOSE
One of the most common causes of infertility in adult women is polycystic ovary syndrome (PCOS) which has been identified with symptoms such as chronic hyperandrogenism, anovulation, and polycystic ovaries. Adiponectin modulates steroidogenesis and the expression of ovulation-related genes. Herein, we assessed the effect of AdipoRon (adiponectin agonist) in the PCOS model mice.
METHODS
The PCOS model was induced with letrozole in the adult female mice and the animals received intraperitoneal injection of AdipoRon (5 mg/kg) for 10 days. Expression of CYP11A, CYP17A, and CYP19A genes, StAR protein, and histomorphology of the ovary were evaluated using real-time RT-PCR, western blotting, and histochemistry methods, respectively.
RESULTS
Although administration of letrozole caused an increase in the expression of CYP11A, CYP17A, and StAR and a decrease in the CYP19A1 expression, injection of AdipoRon reversed these changes. Moreover, AdipoRon treatment resulted in an improvement of folliculogenesis and a reduction of cysts compared to the letrozole-treated mice.
CONCLUSION
It is likely that AdipoRon has protective effects on the PCOS through modulation of cytochrome P450-related genes and steroidogenesis but needs further study to be sure.
目的
成年女性不孕的最常见原因之一是多囊卵巢综合征（PCOS），其特征为慢性高雄激素血症、无排卵和多囊卵巢。脂联素调节类固醇生成和排卵相关基因的表达。在此，我们评估了 AdipoRon（脂联素激动剂）在 PCOS 模型小鼠中的作用。
方法
在成年雌性小鼠中用来曲唑诱导 PCOS 模型，并用 AdipoRon（5mg/kg）腹腔注射 10 天。使用实时 RT-PCR、western blot 和组织化学方法分别评估 CYP11A、CYP17A 和 CYP19A 基因、StAR 蛋白和卵巢的组织形态。
结果
尽管来曲唑给药导致 CYP11A、CYP17A 和 StAR 的表达增加，CYP19A1 的表达减少，但 AdipoRon 的注射逆转了这些变化。此外，与来曲唑处理的小鼠相比，AdipoRon 治疗导致卵泡发生改善和囊肿减少。
结论
AdipoRon 通过调节细胞色素 P450 相关基因和类固醇生成对 PCOS 可能具有保护作用，但需要进一步研究以确定。
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