Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 1A, 10-719, Olsztyn-Kortowo, Poland.
Department of Human Physiology and Pathophysiology, School of Medicine, University of Warmia and Mazury in Olsztyn, Warszawska St. 30, 10-082, Olsztyn, Poland.
Sci Rep. 2021 Apr 22;11(1):8698. doi: 10.1038/s41598-021-88103-z.
Visfatin appears to be an energy sensor involved in the regulation of female fertility, which creates a hormonal link integrating the control of energy homeostasis and reproduction. This study evaluates the expression levels of visfatin gene and protein in selected areas of the porcine hypothalamus responsible for gonadotropin-releasing hormone synthesis: the mediobasal hypothalamus (MBH) and preoptic area (POA), and visfatin concentrations in the blood plasma. The tissue samples were harvested from gilts on days 2-3, 10-12, 14-16, and 17-19 of the estrous cycle, and on days 10-11, 12-13, 15-16, 27-28 of pregnancy. Visfatin was localized in the cytoplasm and nucleus of cells creating both studied hypothalamic structures. The study demonstrated that visfatin gene and protein expression in MBH and POA depends on hormonal status related to the phase of the estrous cycle or early pregnancy. Blood plasma concentrations of visfatin during the estrous cycle were higher on days 2-3 in relation to other studied phases of the cycle, while during early pregnancy, the highest visfatin contents were observed on days 12-13. This study demonstrated visfatin expression in the porcine hypothalamus and its dependence on the hormonal milieu related to the estrous cycle and early pregnancy.
内脂素似乎是一种参与调节女性生育能力的能量传感器，它创建了一个激素链接，将能量稳态和生殖的控制整合在一起。本研究评估了内脂素基因和蛋白在负责促性腺激素释放激素合成的猪下丘脑选定区域中的表达水平：中脑基底部（MBH）和视前区（POA），以及血浆中的内脂素浓度。组织样本取自发情周期第 2-3、10-12、14-16 和 17-19 天以及妊娠第 10-11、12-13、15-16 和 27-28 天的小母猪。内脂素定位于细胞质和细胞核中，形成了这两种研究中的下丘脑结构。研究表明，MBH 和 POA 中的内脂素基因和蛋白表达取决于与发情周期或早期妊娠阶段相关的激素状态。发情周期期间的血浆内脂素浓度在第 2-3 天高于其他研究阶段，而在早期妊娠期间，第 12-13 天观察到的内脂素含量最高。本研究证明了猪下丘脑内的内脂素表达及其对与发情周期和早期妊娠相关的激素环境的依赖性。
