Valipour Parisa, Heidarian Esfandiar, Khoshdel Abolfazl, Gholami-Arjenaki Mostafa
Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Iran J Kidney Dis. 2016 Jul;10(4):189-96.
Gentamicin can lead to acute tubular injury and kidney dysfunction. This study aimed to evaluate the effect of Ferulago angulata on kidney function and other markers in rats with gentamicin-induced nephrotoxicity.
Forty-eight male Wistar rats were divided into the following groups: group 1, the controls; group 2, rats receiving gentamicin (120 mg/kg body weight per day, intraperitoneal) for 7 days without treatment; groups 3, 4 and 5, rats receiving gentamicin for 7 days and oral treatment with 200 mg/kg, 400 mg/kg, and 800 mg/kg body weight per day of Ferulago angulate extract, respectively. Measurements included serum levels of creatinine, urea, uric acid, lipids, ferric-reducing antioxidant power, and protein carbonyl; kidney and serum levels of malondialdehyde; and serum and renal levels of tumor necrosis factor-α. Histopathology of kidney tissue was examined as well as renal catalase, superoxide dismutase, and vitamin C.
Compared to treatment with gentamicin only, treatment with Ferulago angulata resulted in a significantly higher high-density lipoprotein cholesterol, ferric-reducing antioxidant plasma, renal catalase, superoxide dismutase, and vitamin C levels. It was also associated with significantly lower serum levels of creatinine, urea, uric acid, malondialdehyde, protein carbonyl, tumor necrosis factor-α, total cholesterol, triglyceride, low-density lipoprotein cholesterol, and very low-density lipoprotein cholesterol. Ferulago angulate was linked with a lower renal gene expression of tumor necrosis factor-α.
The present study suggests that Ferulago angulate extract has protective effects against nephrotoxicity due to gentamicin.
庆大霉素可导致急性肾小管损伤和肾功能障碍。本研究旨在评估棱角阿魏对庆大霉素诱导的肾毒性大鼠肾功能及其他指标的影响。
48只雄性Wistar大鼠分为以下几组：第1组为对照组；第2组为接受庆大霉素（每天120mg/kg体重，腹腔注射）7天且未接受治疗的大鼠；第3、4和5组为接受庆大霉素7天并分别口服200mg/kg、400mg/kg和800mg/kg体重/天棱角阿魏提取物的大鼠。检测指标包括血清肌酐、尿素、尿酸、血脂、铁还原抗氧化能力和蛋白质羰基水平；肾脏和血清丙二醛水平；以及血清和肾脏肿瘤坏死因子-α水平。检查了肾组织的组织病理学以及肾脏过氧化氢酶、超氧化物歧化酶和维生素C水平。
与仅用庆大霉素治疗相比，棱角阿魏治疗使高密度脂蛋白胆固醇、铁还原抗氧化血浆、肾脏过氧化氢酶、超氧化物歧化酶和维生素C水平显著升高。它还与血清肌酐、尿素、尿酸、丙二醛、蛋白质羰基、肿瘤坏死因子-α、总胆固醇、甘油三酯、低密度脂蛋白胆固醇和极低密度脂蛋白胆固醇水平显著降低有关。棱角阿魏与肿瘤坏死因子-α的肾脏基因表达降低有关。
本研究表明，棱角阿魏提取物对庆大霉素所致肾毒性具有保护作用。
