Boroushaki Mohammad Taher, Fanoudi Sahar, Mollazadeh Hamid, Boroumand-Noughabi Samaneh, Hosseini Azar
Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Physiology and Pharmacology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran.
Iran J Basic Med Sci. 2019 Mar;22(3):328-333. doi: 10.22038/ijbms.2019.31552.7597.
Gentamicin belongs to the family of aminoglycoside antibiotics and is a preferred drug in developing countries because of its low cost, availability, and potent effects against bacterial. However, gentamicin can induce nephrotoxicity. In this research, hydroalcoholic extract of was used against gentamicin- induced nephrotoxicity and its effect against gentamicin-induced nephrotoxicity in rats has been investigated.
The rats were placed into one of these groups: saline group, gentamicin group that received gentamicin 80 mg/kg/day for six days, and two treatment groups that received intraperitoneally at doses of 100 and 200 mg/kg body weight, respectively, 1 hr before gentamicin injections. Urine samples were collected at 24 hr to measure glucose and protein concentration. Blood samples were collected to determine serum urea and creatinine. One kidney was homogenized to measure malondialdehyde and thiol, and the other kidney was kept for pathological studies.
Gentamicin increased the level of urinary glucose and protein, and increased malondialdehyde while it decreased thiol in kidney tissue, and increased the concentration of urea and creatinine in the serum. Histopathological pathology revealed renal damage following gentamicin usage; however, the extract was able to improve gentamicin toxicity.
has positive effects in the attenuation of gentamicin-induced nephrotoxicity.
庆大霉素属于氨基糖苷类抗生素家族，因其成本低、易于获取且对细菌有强效作用，在发展中国家是一种首选药物。然而，庆大霉素可诱发肾毒性。在本研究中，[提取物名称]的水醇提取物被用于对抗庆大霉素诱导的肾毒性，并对其在大鼠中对抗庆大霉素诱导的肾毒性的作用进行了研究。
将大鼠分为以下几组：生理盐水组、接受80mg/kg/天庆大霉素连续6天的庆大霉素组，以及两个治疗组，分别在注射庆大霉素前1小时腹腔注射100和200mg/kg体重的[提取物名称]。在24小时时收集尿液样本以测量葡萄糖和蛋白质浓度。采集血液样本以测定血清尿素和肌酐。将一个肾脏匀浆以测量丙二醛和硫醇，另一个肾脏留作病理研究。
庆大霉素增加了尿葡萄糖和蛋白质水平，增加了丙二醛含量，同时降低了肾组织中的硫醇含量，并增加了血清中尿素和肌酐的浓度。组织病理学显示使用庆大霉素后肾脏受损；然而，该提取物能够改善庆大霉素毒性。
[提取物名称]在减轻庆大霉素诱导的肾毒性方面具有积极作用。
