Rodriguez R J, Arunachalam T A, Parks L W, Caspi E
Department of Microbiology, Oregon State University, 97331-3804, Corvallis, OR.
The Worcester Foundation for Experimental Biology, 222 Maple Avenue, 01545, Shrewsbury, MA.
Lipids. 1983 Nov;18(11):772-5. doi: 10.1007/BF02534634.
A number of cholesterol derivatives were analyzed for their ability to satisfy bulk membrane and high-specificity sparking requirements of a yeast sterol auxotroph (RD5-R) (Rodriguez, R. J., Taylor, F. R., and Parks, L. W. [1982], Biochem. Biophys. Res. Commun. 106, 435-441). Substitution of hydrogen by bromine or iodine at C-26 or substitution of C26-methyl by bromine enabled the resulting sterol to satisfy bulk or sparking functions. The presence of a side-chain hydroxyl or keto group at C-25 on a 26-norcholesterol completely abolished the ability of cholesterol to satisfy either sterol requirement. Growth studies revealed that, while the oxygenated cholesterol derivatives were not growth-supportive of RD5-R, they were not growth-inhibitory.
分析了多种胆固醇衍生物满足酵母甾醇营养缺陷型（RD5-R）（罗德里格斯，R. J.，泰勒，F. R.，和帕克斯，L. W. [1982]，《生物化学与生物物理研究通讯》106，435 - 441）的大量膜和高特异性引发需求的能力。在C-26位用溴或碘取代氢，或用溴取代C26-甲基，能使所得甾醇满足大量或引发功能。26-降胆固醇的C-25位存在侧链羟基或酮基完全消除了胆固醇满足任何一种甾醇需求的能力。生长研究表明，虽然氧化胆固醇衍生物对RD5-R没有生长支持作用，但也没有生长抑制作用。
