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Quantification of collagen ultrastructure after penetrating keratoplasty - implications for corneal biomechanics.穿透性角膜移植术后胶原超微结构的定量分析 - 对角膜生物力学的影响。
PLoS One. 2013 Jul 5;8(7):e68166. doi: 10.1371/journal.pone.0068166. Print 2013.
4
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Invest Ophthalmol Vis Sci. 2012 Mar 15;53(3):1381-7. doi: 10.1167/iovs.11-8524. Print 2012 Mar.
5
Anisotropy of chemical bonds in collagen molecules studied by X-ray absorption near-edge structure (XANES) spectroscopy.胶原分子中化学键的各向异性通过 X 射线吸收近边结构（XANES）光谱研究。
ACS Chem Biol. 2012 Mar 16;7(3):476-80. doi: 10.1021/cb200260d. Epub 2011 Dec 27.
6
3D collagen orientation study of the human cornea using X-ray diffraction and femtosecond laser technology.利用X射线衍射和飞秒激光技术对人角膜进行三维胶原取向研究。
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7
Effects on collagen orientation in the cornea after trephine injury.环钻损伤后对角膜中胶原蛋白排列的影响。
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The structure and function of the limbal stem cell and the disease states associated with limbal stem cell deficiency.角膜缘干细胞的结构与功能以及与角膜缘干细胞缺乏相关的疾病状态。
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Age-related changes of human limbus on in vivo confocal microscopy.人角膜缘在活体共聚焦显微镜下的年龄相关变化。
Cornea. 2008 Aug;27(7):782-6. doi: 10.1097/ICO.0b013e31816f5ec3.
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Circular polarization biomicroscopy: a method for determining human corneal stromal lamellar organization in vivo.圆偏振生物显微镜检查法：一种用于在体内确定人角膜基质层状结构的方法。
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在人体离体组织中识别沃格特栅栏。

Identifying the Palisades of Vogt in Human Ex Vivo Tissue.

作者信息

Sigal Ian A, Steele Jessica, Drexler Scott, Lathrop Kira L

机构信息

University of Pittsburgh School of Medicine, Department of Ophthalmology, Pittsburgh, Pennsylvania, USA; University of Pittsburgh Swanson School of Engineering, Department of Bioengineering, Pittsburgh, Pennsylvania, USA.

University of Pittsburgh School of Medicine, Department of Ophthalmology, Pittsburgh, Pennsylvania, USA.

出版信息

Ocul Surf. 2016 Oct;14(4):435-439. doi: 10.1016/j.jtos.2016.07.003. Epub 2016 Aug 9.

DOI:10.1016/j.jtos.2016.07.003
PMID:27520448
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5155510/
Abstract

PURPOSE

The Palisades of Vogt (POV) constitute the corneal epithelial stem cell niche, but identification of this region in ex vivo tissue is difficult. Here we introduce a simple, direct method of identifying the POV in unsectioned, ex vivo human tissue.

METHODS

Twenty-two eyes were studied, four whole and eighteen rims. Orientation of whole eyes was determined and the eyes were marked to maintain their cardinal orientation prior to dissection. Samples were imaged with brightfield, linearly polarized light and transmitted circularly polarized light (CPL), and optical coherence tomography (OCT) volumes were acquired in all twelve clock hrs around the limbus. Five samples were also fluorescently labeled to identify the epithelial basement membrane, and whole mounts were imaged with laser scanning confocal microscopy. Images were compared to confirm that the structures visible with polarized light were POV.

RESULTS

Under CPL the POV presented as amber radial ridges visible in the superior and inferior regions of the tissue. Identification of POV was confirmed by correlating the structures seen under CPL, OCT and laser-scanning confocal microscopy.

CONCLUSIONS

CPL can be used to quickly identify POV regions in donor tissue. This technique can assist in targeted harvesting of stem cell regions for research and tissue for limbal transplant.

摘要

目的

沃格特栅栏（POV）构成角膜上皮干细胞龛，但在体外组织中识别该区域具有挑战性。在此，我们介绍一种简单、直接的方法来识别未切片的体外人组织中的POV。

方法

研究了22只眼睛，4只完整的和18只边缘组织。确定完整眼睛的方位，并在解剖前对眼睛进行标记以保持其基本方位。样本用明场、线性偏振光和透射圆偏振光（CPL）成像，并在角膜缘周围的所有12个钟点获取光学相干断层扫描（OCT）容积数据。还对5个样本进行荧光标记以识别上皮基底膜，并用激光扫描共聚焦显微镜对整装标本进行成像。比较图像以确认偏振光下可见的结构为POV。

结果

在CPL下，POV呈现为在组织的上部和下部区域可见的琥珀色放射状嵴。通过关联CPL、OCT和激光扫描共聚焦显微镜下看到的结构，证实了POV的识别。

结论

CPL可用于快速识别供体组织中的POV区域。该技术有助于有针对性地采集干细胞区域用于研究和角膜缘移植组织。