糖皮质激素对大鼠嗜铬细胞瘤连续细胞系中酪氨酸羟化酶的诱导作用。
Glucocorticoid induction of tyrosine hydroxylase in a continous cell line of rat pheochromocytoma.
作者信息
Goodman R, Edgar D, Thoenen H, Wechsler W, Herschman H
出版信息
J Cell Biol. 1978 Jul;78(1):R1-7. doi: 10.1083/jcb.78.1.r1.
Abstract
We have established a continous cell line (G1) in which the tyrosine hydroxylase specific activity is increased as much as 50-100-fold in response to dexamethasone. This response is specific for the glucocorticoid class of steroid hormones; it is elicited by dexamethasone, corticosterone, and triamcinolone, but not by estradiol, testosterone, progesterone, or deoxycorticosterone acetate. The increase in tyrosine hydroxylase specific activity is likely to be due to the increased synthesis of new enzyme protein rather than an activation of existing protein molecules, inasmuch as this increase is completely blocked by cycloheximide.
摘要
我们已经建立了一个连续细胞系（G1），其中酪氨酸羟化酶的比活性在对地塞米松的反应中增加了50至100倍。这种反应对糖皮质激素类甾体激素具有特异性；地塞米松、皮质酮和曲安西龙能引发该反应，但雌二醇、睾酮、孕酮或醋酸脱氧皮质酮则不能。酪氨酸羟化酶比活性的增加可能是由于新酶蛋白合成增加，而非现有蛋白质分子的激活，因为这种增加被环己酰亚胺完全阻断。
相似文献
1
Glucocorticoid induction of tyrosine hydroxylase in a continous cell line of rat pheochromocytoma.糖皮质激素对大鼠嗜铬细胞瘤连续细胞系中酪氨酸羟化酶的诱导作用。
J Cell Biol. 1978 Jul;78(1):R1-7. doi: 10.1083/jcb.78.1.r1.
2
Induction of tyrosine hydroxylase by cyclic AMP and glucocorticoids in a rat pheochromocytoma cell line: effect of the inducing agents alone or in combination on the enzyme levels and rate of synthesis of tyrosine hydroxylase.环磷酸腺苷（cAMP）和糖皮质激素对大鼠嗜铬细胞瘤细胞系酪氨酸羟化酶的诱导作用：单独或联合诱导剂对酪氨酸羟化酶水平及合成速率的影响
Mol Pharmacol. 1986 Nov;30(5):486-96.
3
Induction of mRNA for tyrosine hydroxylase by cyclic AMP and glucocorticoids in a rat pheochromocytoma cell line: evidence for the regulation of tyrosine hydroxylase synthesis by multiple mechanisms in cells exposed to elevated levels of both inducing agents.环磷酸腺苷（cAMP）和糖皮质激素在大鼠嗜铬细胞瘤细胞系中诱导酪氨酸羟化酶mRNA的表达：在同时暴露于两种诱导剂高水平的细胞中，酪氨酸羟化酶合成受多种机制调控的证据。
Mol Pharmacol. 1986 Nov;30(5):497-503.
4
Nerve growth factor-mediated induction of tyrosine hydroxylase in a clonal pheochromocytoma cell line.神经生长因子介导的克隆性嗜铬细胞瘤细胞系中酪氨酸羟化酶的诱导
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4587-90. doi: 10.1073/pnas.75.9.4587.
5
Translation of tyrosine hydroxylase from poly(A)-mRNA in pheochromocytoma cells is enhanced by dexamethasone.在嗜铬细胞瘤细胞中，地塞米松可增强酪氨酸羟化酶从多聚腺苷酸信使核糖核酸（poly(A)-mRNA）的翻译。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):1269-73. doi: 10.1073/pnas.78.2.1269.
6
Nerve growth factor-mediated stimulation of tyrosine hydroxylase activity in a clonal rat pheochromocytoma cell line.神经生长因子介导的对大鼠嗜铬细胞瘤克隆细胞系中酪氨酸羟化酶活性的刺激作用。
Brain Res. 1981 Oct 19;222(2):225-33. doi: 10.1016/0006-8993(81)91029-5.
7
8
Permissive action of glucocorticoids in induction of tyrosine hydroxylase by nerve growth factor in a pheochromocytoma cell line.糖皮质激素在嗜铬细胞瘤细胞系中对神经生长因子诱导酪氨酸羟化酶的允许作用。
Brain Res. 1980 Jul 7;193(1):304-8. doi: 10.1016/0006-8993(80)90972-5.
9
Effect of dexamethasone on biopterin levels and tyrosine hydroxylase activity in PC-12 cells.地塞米松对PC-12细胞中生物蝶呤水平和酪氨酸羟化酶活性的影响。
Biochem Pharmacol. 1984 Aug 1;33(15):2361-6. doi: 10.1016/0006-2952(84)90706-8.
10
Selective enzyme induction in a nerve growth factor-responsive pheochromocytoma cell line (PC 12).在一种对神经生长因子有反应的嗜铬细胞瘤细胞系（PC 12）中的选择性酶诱导。
Brain Res. 1978 Oct 6;154(1):186-90. doi: 10.1016/0006-8993(78)91070-3.
引用本文的文献
1
Management and Long-Term Monitoring of a Young Patient with Pheochromocytoma and RET Mutation: A Case Report.一名患有嗜铬细胞瘤和RET突变的年轻患者的管理与长期监测：病例报告
Int Med Case Rep J. 2025 Mar 24;18:395-404. doi: 10.2147/IMCRJ.S504562. eCollection 2025.
2
Ectopic ACTH-producing neuroendocrine tumor occurring with large recurrent metastatic pheochromocytoma: a case report.伴大复发转移嗜铬细胞瘤的异位 ACTH 分泌型神经内分泌肿瘤：病例报告。
BMC Endocr Disord. 2022 Jul 19;22(1):184. doi: 10.1186/s12902-022-01090-8.
3
Effects of Trilostane on urinary Catecholamines and their metabolites in dogs with Hypercortisolism.曲洛司坦对高皮质醇血症犬尿儿茶酚胺及其代谢产物的影响。
BMC Vet Res. 2017 Sep 4;13(1):279. doi: 10.1186/s12917-017-1187-0.
4
An unusual presentation of pheochromocytoma.嗜铬细胞瘤的一种不寻常表现。
NDT Plus. 2011 Oct;4(5):331-4. doi: 10.1093/ndtplus/sfr055. Epub 2011 May 6.
5
Pheochromocytoma crisis after a dexamethasone suppression test for adrenal incidentaloma.促肾上腺皮质激素抑制试验后发生的意外肾上腺瘤的嗜铬细胞瘤危象。
Endocrine. 2010 Feb;37(1):213-9. doi: 10.1007/s12020-009-9303-y. Epub 2010 Jan 5.
6
Novel basic and clinical aspects of congenital adrenal hyperplasia.先天性肾上腺皮质增生症的新基础与临床研究进展
Rev Endocr Metab Disord. 2001 Aug;2(3):289-96. doi: 10.1023/a:1011520600476.
7
A quantitative electron microscopic study of the effect of glucocorticoids in vivo on the early postnatal differentiation of paraneuronal cells in the carotid body and the adrenal medulla of the rat.一项关于糖皮质激素在体内对大鼠颈动脉体和肾上腺髓质旁神经细胞出生后早期分化影响的定量电子显微镜研究。
Anat Embryol (Berl). 1986;174(3):307-19. doi: 10.1007/BF00698781.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
EFFECT OF DENERVATION AND ENZYME INHIBITION ON DOPA DECARBOXYLASE AND MONOAMINE OXIDASE ACTIVITIES OF RAT PINEAL GLAND.去神经支配和酶抑制对大鼠松果体多巴脱羧酶和单胺氧化酶活性的影响。
J Neurochem. 1965 May;12:417-29. doi: 10.1111/j.1471-4159.1965.tb04242.x.
3
A new class of tyrosine hydroxylase inhibitors and a simple assay of inhibition in vivo.一类新型酪氨酸羟化酶抑制剂及一种简单的体内抑制测定法。
Biochem Pharmacol. 1967 Jul 7;16(7):1313-21. doi: 10.1016/0006-2952(67)90162-1.
4
Increase in tyrosine hydroxylase activity after reserpine administration.利血平给药后酪氨酸羟化酶活性增加。
J Pharmacol Exp Ther. 1969 Sep;169(1):74-9.
5
Establishment of functional clonal lines of neurons from mouse neuroblastoma.从小鼠神经母细胞瘤建立功能性神经元克隆系。
Proc Natl Acad Sci U S A. 1969 Sep;64(1):311-5. doi: 10.1073/pnas.64.1.311.
6
Pheochromocytoma.嗜铬细胞瘤
Cancer. 1972 Feb;29(2):327-31. doi: 10.1002/1097-0142(197202)29:2<327::aid-cncr2820290210>3.0.co;2-3.
7
Differentiated rat glial cell strain in tissue culture.组织培养中的分化大鼠神经胶质细胞系。
Science. 1968 Jul 26;161(3839):370-1. doi: 10.1126/science.161.3839.370.
8
Epidermal growth factor and a new derivative. Rapid isolation procedures and biological and chemical characterization.表皮生长因子及一种新衍生物。快速分离方法以及生物学和化学特性
J Biol Chem. 1972 Dec 10;247(23):7609-11.
9
In vitro differentiation of a mouse neuroblastoma.小鼠神经母细胞瘤的体外分化
Proc Natl Acad Sci U S A. 1969 Sep;64(1):316-23. doi: 10.1073/pnas.64.1.316.
10
Selection for neuroblastoma cells that synthesize certain transmitters.筛选合成特定递质的神经母细胞瘤细胞。
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2530-3. doi: 10.1073/pnas.71.6.2530.
Zotero 插件