共聚焦显微镜下苏木精和伊红图像信息采集与优化方法
Methods of Hematoxylin and Erosin Image Information Acquisition and Optimization in Confocal Microscopy.
作者信息
Yoon Woong Bae, Kim Hyunjin, Kim Kwang Gi, Choi Yongdoo, Chang Hee Jin, Sohn Dae Kyung
机构信息
Biomedical Engineering Branch, Research Institute and Hospital, National Cancer Center, Goyang, Korea.
Molecular Imaging & Therapy Branch, Research Institute and Hospital, National Cancer Center, Goyang, Korea.
出版信息
Healthc Inform Res. 2016 Jul;22(3):238-42. doi: 10.4258/hir.2016.22.3.238. Epub 2016 Jul 31.
OBJECTIVES
We produced hematoxylin and eosin (H&E) staining-like color images by using confocal laser scanning microscopy (CLSM), which can obtain the same or more information in comparison to conventional tissue staining.
METHODS
We improved images by using several image converting techniques, including morphological methods, color space conversion methods, and segmentation methods.
RESULTS
An image obtained after image processing showed coloring very similar to that in images produced by H&E staining, and it is advantageous to conduct analysis through fluorescent dye imaging and microscopy rather than analysis based on single microscopic imaging.
CONCLUSIONS
The colors used in CLSM are different from those seen in H&E staining, which is the method most widely used for pathologic diagnosis and is familiar to pathologists. Computer technology can facilitate the conversion of images by CLSM to be very similar to H&E staining images. We believe that the technique used in this study has great potential for application in clinical tissue analysis.
目的
我们利用共聚焦激光扫描显微镜（CLSM）生成了苏木精和伊红（H&E）染色样彩色图像，与传统组织染色相比，CLSM能够获取相同或更多信息。
方法
我们通过使用多种图像转换技术来改善图像，包括形态学方法、颜色空间转换方法和分割方法。
结果
图像处理后获得的图像显示出与H&E染色产生的图像非常相似的着色效果，并且通过荧光染料成像和显微镜进行分析比基于单一显微镜成像的分析更具优势。
结论
CLSM中使用的颜色与H&E染色中所见的颜色不同，H&E染色是病理诊断中使用最广泛且病理学家熟悉的方法。计算机技术可以促进CLSM图像转换为与H&E染色图像非常相似的图像。我们认为本研究中使用的技术在临床组织分析中具有巨大的应用潜力。
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