Sutherland Megan R, Béland Chanel, Lukaszewski Marie-Amélie, Cloutier Anik, Bertagnolli Mariane, Nuyt Anne Monique
Sainte-Justine University Hospital and Research Center, Montreal, Quebec, Canada Department of Pediatrics, Université de Montréal, Montreal, Quebec, Canada.
Sainte-Justine University Hospital and Research Center, Montreal, Quebec, Canada Department of Pediatrics, Université de Montréal, Montreal, Quebec, Canada
Physiol Rep. 2016 Aug;4(15). doi: 10.14814/phy2.12887.
Preterm neonates are prematurely exposed to high oxygen levels at birth which may adversely impact ongoing renal development. The aim of this study was to determine the effects of neonatal hyperoxia exposure on renal function and morphology with aging. Sprague Dawley rat pups were raised in a hyperoxic environment (80% oxygen) from P3 to P10 during ongoing postnatal nephrogenesis. Control litters were kept in room air (n = 6-8 litters/group; one male, one female/litter/age). Kidney function (urine and plasma creatinine, sodium, and protein) and morphology (renal corpuscle size, glomerulosclerosis, fibrosis, and glomerular crescents) were assessed at 1, 5, and 11 months of age. Neonatal hyperoxia exposure had no impact on body or kidney weights. Creatinine clearance was significantly reduced following hyperoxia exposure at 5 months; there was no significant effect on renal function at 1 or 11 months. The percentage of crescentic glomeruli (indicative of glomerular injury) was markedly increased in 11 month hyperoxia-exposed males. Renal corpuscle size, glomerulosclerosis index, and renal fibrosis were not affected. Findings suggest that exposure to high oxygen levels during development may impact renal functional capacity and increase susceptibility to renal disease in adulthood depending on age and sex.
早产新生儿在出生时过早暴露于高氧环境，这可能会对其正在进行的肾脏发育产生不利影响。本研究的目的是确定新生儿高氧暴露对肾脏功能和形态随年龄增长的影响。在出生后持续的肾发生过程中，将斯普拉格-道利大鼠幼崽从出生后第3天（P3）至第10天（P10）置于高氧环境（80%氧气）中饲养。对照组幼崽置于室内空气中（每组6 - 8窝；每窝/每个年龄一只雄性、一只雌性）。在1、5和11月龄时评估肾脏功能（尿液和血浆肌酐、钠和蛋白质）和形态（肾小体大小、肾小球硬化、纤维化和肾小球新月体）。新生儿高氧暴露对体重或肾脏重量没有影响。高氧暴露5个月后肌酐清除率显著降低；在1个月或11个月时对肾功能没有显著影响。在11月龄高氧暴露的雄性大鼠中，新月形肾小球的百分比（表明肾小球损伤）明显增加。肾小体大小、肾小球硬化指数和肾纤维化不受影响。研究结果表明，发育过程中暴露于高氧水平可能会影响肾脏功能，并根据年龄和性别增加成年后患肾脏疾病的易感性。
