Coelho Miguel B, Ascher David B, Gooding Clare, Lang Emma, Maude Hannah, Turner David, Llorian Miriam, Pires Douglas E V, Attig Jan, Smith Christopher W J
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, U.K.
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, U.K. Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima 1715, Belo Horizonte 30190-002, Brazil.
Biochem Soc Trans. 2016 Aug 15;44(4):1058-65. doi: 10.1042/BST20160080.
Polypyrimidine tract binding protein (PTBP1) is a heterogeneous nuclear ribonucleoprotein (hnRNP) that plays roles in most stages of the life-cycle of pre-mRNA and mRNAs in the nucleus and cytoplasm. PTBP1 has four RNA binding domains of the RNA recognition motif (RRM) family, each of which can bind to pyrimidine motifs. In addition, RRM2 can interact via its dorsal surface with proteins containing short peptide ligands known as PTB RRM2 interacting (PRI) motifs, originally found in the protein Raver1. Here we review our recent progress in understanding the interactions of PTB with RNA and with various proteins containing PRI ligands.
聚嘧啶序列结合蛋白（PTBP1）是一种不均一核核糖核蛋白（hnRNP），在细胞核和细胞质中前体mRNA和mRNA生命周期的大多数阶段发挥作用。PTBP1具有RNA识别基序（RRM）家族的四个RNA结合结构域，每个结构域都能与嘧啶基序结合。此外，RRM2可通过其背面与含有短肽配体的蛋白质相互作用，这些短肽配体被称为PTB RRM2相互作用（PRI）基序，最初在蛋白质Raver1中发现。在此，我们综述了我们在理解PTB与RNA以及与各种含有PRI配体的蛋白质之间相互作用方面的最新进展。
