Gutierrez M Gertrude, Jalali-Yazdi Farzad, Peruzzi Justin, Riche Carson T, Roberts Richard W, Malmstadt Noah
Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, 925 Bloom Walk, Los Angeles, CA, 90089, USA.
Department of Chemical Engineering, University of Virginia, 102 Engineers' Way, P.O. Box 400741, Charlottesville, VA, 22904-4741, USA.
Small. 2016 Oct;12(38):5256-5260. doi: 10.1002/smll.201601540. Epub 2016 Aug 16.
G protein-coupled receptor (GPCR) is incorporated into polymeric vesicles made up of diblock copolymer bilayers. Successfully incorporated GPCRs exhibit correct biased physiological orientation and respond to various ligands. After extended dehydrated storage via lyophilization and subsequent rehydration, diblock copolymer polymersomes retain their shape and incorporated GPCR retains its function.
G蛋白偶联受体(GPCR)被整合到由双嵌段共聚物双层组成的聚合物囊泡中。成功整合的GPCR表现出正确的偏向生理取向,并对各种配体作出反应。通过冻干进行长时间脱水储存并随后再水化后,双嵌段共聚物聚合物囊泡保持其形状,且整合的GPCR保持其功能。