Gutierrez M Gertrude, Malmstadt Noah
Mork Family Department of Chemical Engineering and Materials Science, University of Southern California , 925 Bloom Walk, Los Angeles, California 90089, United States.
J Am Chem Soc. 2014 Oct 1;136(39):13530-3. doi: 10.1021/ja507221m. Epub 2014 Sep 19.
We demonstrate successful incorporation of the G protein coupled receptor 5-HT1A into giant unilamellar vesicles using an agarose rehydration method. With direct observation using fluorescence techniques, we report preferential segregation of 5-HT1A into the cholesterol-poor liquid disordered phase of the membrane, contradicting previous reports of lipid raft segregation. Furthermore, altering the concentration of cholesterol and sphingomyelin in ternary mixtures does not alter 5-HT1A segregation into the liquid disordered phase.
我们证明了利用琼脂糖复水法成功地将G蛋白偶联受体5-HT1A整合到巨型单层囊泡中。通过荧光技术直接观察，我们报告5-HT1A优先分离到膜的胆固醇含量低的液态无序相中，这与之前关于脂筏分离的报道相矛盾。此外，改变三元混合物中胆固醇和鞘磷脂的浓度并不会改变5-HT1A向液态无序相的分离。
