Gertrude Gutierrez M, Yoshida Shotaro, Malmstadt Noah, Takeuchi Shoji
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, Japan.
APL Bioeng. 2018 Jan 2;2(1):016104. doi: 10.1063/1.5002604. eCollection 2018 Mar.
Using traditional 2-D photolithographic methods, surface patterns are made on agarose and used to form lipid vesicles with controlled size and layout. Depending on the size and layout of the patterned structures, the lipid bilayer vesicle size can be tuned and placement can be predetermined. Vesicles formed on 2-D patterned surfaces can be harvested for further investigations or can be assayed directly on the patterned surface. Lipid vesicles on the patterned surface are assayed for unilamellarity and protein incorporation, and vesicles are indeed unilamellar as observed from outer leaflet fluorescence quenching. Vesicles successfully incorporate the integral membrane protein α-hemolysin and maintain its membrane transport function.
使用传统的二维光刻方法，在琼脂糖上制作表面图案，并用于形成具有可控大小和布局的脂质囊泡。根据图案化结构的大小和布局，可以调整脂质双层囊泡的大小，并预先确定其位置。在二维图案化表面上形成的囊泡可以收集用于进一步研究，也可以直接在图案化表面上进行检测。对图案化表面上的脂质囊泡进行单层性和蛋白质掺入检测，从外叶荧光淬灭观察到囊泡确实是单层的。囊泡成功地掺入了整合膜蛋白α-溶血素并维持其膜转运功能。
